Overview

  • Product nameAnti-MPPE1 antibody
    See all MPPE1 primary antibodies
  • Description
    Rabbit polyclonal to MPPE1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 108-157 (LLQPEVVFIL GDIFDEGKWS TPEAWADDVE RFQKMFRHPS HVQLKVVAGN) of Human MPPE1 (NP_075563).

  • Positive control
    • Transfected 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceThe specific function of metallophosphoesterase 1 (MPPE1) is not yet known. There are 5 isoforms produced by alternative splicing which have molecular weights of 45 kDa, 39 kDa (2 isoforms), 38 kDa and 29 kDa.
  • Cellular localizationEndoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. Golgi apparatus, cis-Golgi network membrane; Multi-pass membrane protein. Note: Also localizes to endoplasmic reticulum exit site.
  • Database links
  • Alternative names
    • EC 3.1.-.- antibody
    • Metallo phosphoesterase antibody
    • Metallophosphoesterase 1 antibody
    • PGAP5 antibody
    • Post-GPI attachment to proteins factor 5 antibody
    see all

Anti-MPPE1 antibody images

  • Anti-MPPE1 antibody (ab87298) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : ~40 kDa (why is the actual band size different from the predicted?)

References for Anti-MPPE1 antibody (ab87298)

ab87298 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"