Overview

  • Product name
  • Description
    Rabbit polyclonal to MPRA
  • Specificity
    This antibody detects endogenous levels of total MPRA protein.
  • Tested applications
    Suitable for: ICC, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from an internal region of human MPRA.

  • Positive control
    • Extracts from 293 and COLO cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.

Target

  • Relevance
    MPRA belongs to the ADIPOR family. It is a steroid membrane receptor which binds progesterone in vitro. MPRA may be involved in oocyte maturation. It is expressed in ovary, testis, placenta, uterus and bladder
  • Cellular localization
    Membrane; Multi pass membrane protein
  • Database links
  • Alternative names
    • Membrane progestin receptor alpha antibody
    • mPR alpha antibody
    • MRPA antibody
    • mSR antibody
    • PAQR7 antibody
    • PGLP antibody
    • Progestin and adipoQ receptor family member VII antibody
    see all

Anti-MPRA antibody images

  • All lanes : Anti-MPRA antibody (ab75508) at 1/500 dilution

    Lane 1 : Extracts from COLO cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from 293 cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • ab75508 staining MPRA in murine ovary tissue sections by Immunohistochemistry (PFA-fixed).
    Tissue was fixed in paraformaldehyde, blocked using 10% normal goat serum for 1 hour at 25°C and then incubated with ab75508 at a 1/500 dilution for 12 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

    Procedures were tested with and without a permeabilization step and better results were acquired by eliminating the permeabilization step, due to PAQR7 being expressed on the cell membrane.

    See Abreview

References for Anti-MPRA antibody (ab75508)

This product has been referenced in:
  • Bashour NM & Wray S Progesterone directly and rapidly inhibits GnRH neuronal activity via progesterone receptor membrane component 1. Endocrinology 153:4457-69 (2012). Read more (PubMed: 22822163) »

See 1 Publication for this product

Product Wall



It is catalog number ab171582.

Application
Immunocytochemistry
Sample
Mouse Cell (Mouse ovary sections)
Specification
Mouse ovary sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. Nicholas Bashour

Verified customer

Submitted Dec 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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