Overview

  • Product name
  • Description
    Rabbit polyclonal to MPRA
  • Specificity
    This antibody detects endogenous levels of total MPRA protein.
  • Tested applications
    Suitable for: ICC, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from an internal region of human MPRA.

  • Positive control
    • Extracts from 293 and COLO cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.

Target

  • Relevance
    MPRA belongs to the ADIPOR family. It is a steroid membrane receptor which binds progesterone in vitro. MPRA may be involved in oocyte maturation. It is expressed in ovary, testis, placenta, uterus and bladder
  • Cellular localization
    Membrane; Multi pass membrane protein
  • Database links
  • Alternative names
    • Membrane progestin receptor alpha antibody
    • mPR alpha antibody
    • MRPA antibody
    • mSR antibody
    • PAQR7 antibody
    • PGLP antibody
    • Progestin and adipoQ receptor family member VII antibody
    see all

Images

  • All lanes : Anti-MPRA antibody (ab75508) at 1/500 dilution

    Lane 1 : Extracts from COLO cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from 293 cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • ab75508 staining MPRA in murine ovary tissue sections by Immunohistochemistry (PFA-fixed).
    Tissue was fixed in paraformaldehyde, blocked using 10% normal goat serum for 1 hour at 25°C and then incubated with ab75508 at a 1/500 dilution for 12 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

    Procedures were tested with and without a permeabilization step and better results were acquired by eliminating the permeabilization step, due to PAQR7 being expressed on the cell membrane.

    See Abreview

References

This product has been referenced in:
  • Bashour NM & Wray S Progesterone directly and rapidly inhibits GnRH neuronal activity via progesterone receptor membrane component 1. Endocrinology 153:4457-69 (2012). Read more (PubMed: 22822163) »

See 1 Publication for this product

Customer reviews and Q&As



It is catalog number ab171582.

Application
Immunocytochemistry
Sample
Mouse Cell (Mouse ovary sections)
Specification
Mouse ovary sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. Nicholas Bashour

Verified customer

Submitted Dec 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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