Overview

  • Product name
  • Description
    Rabbit polyclonal to Mps1
  • Specificity
    ab24227 reacts with TTK (MPS1) protein. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide:

    LVGLNSPNSILKAAKTLYEHYSGGESHNSSSSKTFEKKRGKK

    , corresponding to C terminal amino acids 800-841 of Human Mps1

  • Positive control
    • Hela cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab24227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2500 - 1/25000. Predicted molecular weight: 95 kDa.
IP Use at an assay dependent dilution.

Target

  • Function
    Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
  • Tissue specificity
    Present in rapidly proliferating cell lines.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • cancer/testis antigen 96 antibody
    • CT96 antibody
    • Dual specificity protein kinase TTK antibody
    • ECSTY kinase antibody
    • ESK antibody
    • FLJ38280 antibody
    • hMPS1 antibody
    • Monopolar Spindle 1 Like 1 antibody
    • MPH1 antibody
    • Mps 1 antibody
    • MPS1L1 antibody
    • Phosphotyrosine Picked Threonine Kinase antibody
    • Phosphotyrosine picked threonine-protein kinase antibody
    • PYT antibody
    • RP3-357D13.2 antibody
    • ttk antibody
    • TTK Protein Kinase antibody
    • TTK_HUMAN antibody
    see all

Images

  • All lanes : Anti-Mps1 antibody (ab24227)

    Lane 1 : HeLa cell lyste at 15µg and ab at 0.033ug/ml
    Lane 2 : HeLa cell lyste at 50ug and ab at 0.033ug/ml
    Lane 3 : HeLa cell lyste at 50ug and ab at 0.33ug/ml
    Lane 4 : HeLa cell lyste at 50ug and ab at 0.33ug/ml
    Lane 5 : HeLa cell lyste at 2mg and ab at 1ug/2mg lysate
    Lane 6 : HeLa cell lyste at 2mg and ab at 1ug/2mg lysate
    Lane 7 : HeLa cell lyste at 2mg and ab at 1ug/2mg lysate

    Developed using the ECL technique

    Predicted band size : 95 kDa
    Observed band size : 95 kDa
  • ICC/IF image of ab24227 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24227, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab24227 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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