Anti-MR1 antibody (ab55164)

Overview

  • Product nameAnti-MR1 antibody
    See all MR1 primary antibodies
  • Description
    Mouse monoclonal to MR1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: TEPPLVRVNR KETFPGVTAL FCKAHGFYPP EIYMTWMKNG EEIVQEIDYG DILPSGDGTY QAWASIELDP QSSNLYSCHV EHCGVHMVLQ VPQESETIPL , corresponding to amino acids 201-301 of Human MR1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceSimilar to a typical class I MHC molecule, the MR1 (MHC-related 1) protein comprises a signal sequence, 3 extracellular domains (alpha-1, alpha-2, and alpha-3), a transmembrane domain, and a cytoplasmic domain. MR1 encodes peptide-binding domains similar to those encoded by HLA class I genes.
    • Database links
    • Alternative names
      • Class I histocompatibility antigen like protein antibody
      • HLALS antibody
      • Major histocompatibility complex class I like sequence antibody
      • Major histocompatibility complex class I related antibody
      • Major histocompatibility complex class I related gene protein antibody
      • Major histocompatibility complex class I related isoform CRA a antibody
      • Major histocompatibility complex class I related isoform CRA c antibody
      • Major histocompatibility complex class I related isoform CRA d antibody
      • MHC class I like antigen MR 1 antibody
      • MHC class I like antigen MR1 antibody
      • MHC class I related gene protein antibody
      • MHC class I related protein 1 isoform B antibody
      • MHC class I related protein 1 isoform C antibody
      • MR 1 antibody
      • Mr1 protein antibody
      • MR1B antibody
      • MR1C antibody
      see all

    Anti-MR1 antibody images

    • Western blot against tagged recombinant protein immunogen using ab55164 MR1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    References for Anti-MR1 antibody (ab55164)

    ab55164 has not yet been referenced specifically in any publications.

    Product Wall

    Thanks for your enquiry and for including the western blot data.

    By comparing the isotype control blot and the MR1 blots you provided, it appears the low molecular weight bands just under 31kDa are due to reactivity with the secondary antibod...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Human Liver from chronically infected Hepatitis C)
    Specification Human Liver from chronically infected Hepatitis C
    Fixative Formalin Fixed Paraffin Embedded
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EMS R-Buffer A,100 Citrate
    Permeabilization No
    Blocking step Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 17°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 26 2012

    DISCOUNT CODE: 00000

    Expiration date:06. October 2012

    I am very pleased to hear your customer would like to accept our offer and test ab55164 in IHC-Fr and/or IHC-P and humans samples.

    This code will give your customer the ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"