Overview

  • Product name
  • Description
    Mouse polyclonal to MRAP
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Full length protein, corresponding to amino acids 1-172 of Human MRAP (NP_848932.1).

  • Positive control
    • MRAP transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab168120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa.

Target

  • Function
    Required for MC2R expression in certain cell types, suggesting that it is involved in the processing, trafficking or function of MC2R. May be involved in the intracellular trafficking pathways in adipocyte cells.
  • Tissue specificity
    Expressed in adrenal cortex, testis, breast, thyroid, lymph node, ovary and fat. Expressed in adipose tissues.
  • Involvement in disease
    Defects in MRAP are the cause of glucocorticoid deficiency type 2 (GCCD2) [MIM:607398]; also known as familial glucocorticoid deficiency type 2 (FGD2). GCCD2 is an autosomal recessive disorder due to congenital insensitivity or resistance to adrenocorticotropin (ACTH). It is characterized by progressive primary adrenal insufficiency, without mineralocorticoid deficiency.
  • Cellular localization
    Cytoplasm > perinuclear region. Cytoplasm. Cell membrane. Endoplasmic reticulum. Concentrated at the perinuclear membrane region. Upon insulin stimulation, it is redistributed into spotty structures throughout the cytoplasm (By similarity). Localizes both to plasma membrane and endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • B27 antibody
    • C21orf61 antibody
    • FALP antibody
    • Fat cell-specific low molecular weight protein antibody
    • Fat tissue-specific low MW protein antibody
    • FGD2 antibody
    • GCCD2 antibody
    • Melanocortin-2 receptor accessory protein antibody
    • Mrap antibody
    • MRAP_HUMAN antibody
    see all

Images

  • All lanes : Anti-MRAP antibody (ab168120) at 1 µg/ml

    Lane 1 : MRAP transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG

    Predicted band size: 19 kDa

References

This product has been referenced in:
  • Nanba K  et al. H295R expression of melanocortin 2 receptor accessory protein results in ACTH responsiveness. J Mol Endocrinol 56:69-76 (2016). Read more (PubMed: 26576642) »

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Baboon Tissue lysate - whole (fetal adrenal)
Gel Running Conditions
Reduced Denaturing (16.5% Tris tricine gel)
Loading amount
25 µg
Specification
fetal adrenal
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
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Submitted Mar 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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