Overview

  • Product nameAnti-MRas antibody
    See all MRas primary antibodies
  • Description
    Rabbit polyclonal to MRas
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:

    KKKKTKWRGDRATGTHKLQ

    , corresponding to C terminal amino acids 186-204 of Human MRas

  • Positive control
    • HeLa or HEK293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab26303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 24 kDa). Block with 5% milk and dilute in TBST only.
ICC/IF 1/1000.

Target

  • FunctionMay serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.
  • Tissue specificityExpression highly restricted to the brain and heart.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ42964 antibody
    • M ras antibody
    • M-ras antibody
    • Mras antibody
    • Muscle and microspikes Ras antibody
    • Muscle RAS oncogene homolog antibody
    • Muscle Ras oncogene homologue antibody
    • Muscle Ras viral oncogene homolog antibody
    • R ras3 antibody
    • R-ras3 antibody
    • Ras related protein MRas antibody
    • Ras related protein RRas3 antibody
    • Ras-related protein M-Ras antibody
    • Ras-related protein R-Ras3 antibody
    • RASM_HUMAN antibody
    • Related Ras viral oncogene homolog 3 antibody
    • RRas3 antibody
    • XRas antibody
    see all

Anti-MRas antibody images

  • All lanes : Anti-MRas antibody (ab26303)

    Lane 1 : HEK 293T cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : HeLa cell lysate

    Secondary
    anti-rabbit HRP conjugated
    Developed using the ECL technique

    Predicted band size : 24 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab26303 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26303, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab26303 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab26303, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-MRas antibody (ab26303)

ab26303 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab26303.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"