The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/20000.
Flow Cyt: 1/5 (Abreview).
ICC/IF: 1/500 - 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 167 kDa (predicted molecular weight: 167 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs).
Tissue specificityUbiquitous with low expression in brain, placenta, lung, kidney, pancreas, spleen, thymus and colon. Expressed in endothelial cells, fibroblasts and macrophages. Highly expressed in fetal lung and kidney.
DomainC-type lectin domains 3 to 8 are not required for calcium-dependent binding of mannose, fucose and N-acetylglucosamine. C-type lectin domain 2 is responsible for sugar-binding in a calcium-dependent manner. Fibronectin type-II domain mediates collagen-binding. Ricin B-type lectin domain contacts with the second C-type lectin domain.
ab70132, at a 1/50 dilution, staining MRC2 in paraffin embedded human brain tissue by Immunohistochemistry, in the absence or presence of the immunising peptide.
Western blot - MRC2 antibody (ab70132)
All lanes : Anti-MRC2 antibody (ab70132) at 1/500 dilution
Lane 1 : 293 cell extracts Lane 2 : 293 cell extracts with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 167 kDa Observed band size : 167 kDa
Flow Cytometry - MRC2 antibody (ab70132)This image is courtesy of an anonymous Abreview
ab70132 staining IMRC2 in tonsillar tissue by Flow Cytometry. Gating strategy : Viable leukocytes gated in FSC/SSC. The sample was incubated with the primary antibody (dilution 1/5 in PBS/0.5%BSA/2.5mM EDTA), for 30 minutes at 4°C. An FITC-conjugated goat polyclonal to Rabbit IgG (dilution 1/50) was used as secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2 antibody (ab70132)This image is courtesy of an anonymous Abreview
ab70132 staining MRC2 in Human pancreatic cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with Inhibitor CM for 4 minutes at 37°C; antigen retrieval was by heat mediation in Tris/Borate/EDTA buffer pH 8. Samples were incubated with primary antibody (1/50 ) for 32 minutes at 37°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.