Overview

  • Product nameAnti-MRC2 antibody
    See all MRC2 primary antibodies
  • Description
    Rabbit polyclonal to MRC2
  • Specificityab70132 detects endogenous levels of total MRC2 protein.
  • Tested applicationsSuitable for: Flow Cyt, WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human MRC2.

  • Positive control
    • HepG2 cells, 293 cell extracts and human brain tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab70132 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
WB
ELISA
IHC-P
ICC/IF
  • Application notesELISA: 1/20000.
    Flow Cyt: 1/5 (Abreview).
    ICC/IF: 1/500 - 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 167 kDa (predicted molecular weight: 167 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs).
    • Tissue specificityUbiquitous with low expression in brain, placenta, lung, kidney, pancreas, spleen, thymus and colon. Expressed in endothelial cells, fibroblasts and macrophages. Highly expressed in fetal lung and kidney.
    • Sequence similaritiesContains 8 C-type lectin domains.
      Contains 1 fibronectin type-II domain.
      Contains 1 ricin B-type lectin domain.
    • DomainC-type lectin domains 3 to 8 are not required for calcium-dependent binding of mannose, fucose and N-acetylglucosamine. C-type lectin domain 2 is responsible for sugar-binding in a calcium-dependent manner.
      Fibronectin type-II domain mediates collagen-binding.
      Ricin B-type lectin domain contacts with the second C-type lectin domain.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-type lectin domain family 13 member E antibody
      • C-type mannose receptor 2 antibody
      • CD 280 antibody
      • CD280 antibody
      • CD280 antigen antibody
      • CLEC 13E antibody
      • CLEC13E antibody
      • ENDO 180 antibody
      • ENDO180 antibody
      • Endocytic receptor (macrophage mannose receptor family) antibody
      • Endocytic receptor 180 antibody
      • FLJ35911 antibody
      • KIAA0709 antibody
      • Macrophage mannose receptor 2 antibody
      • Mannose receptor C type 2 antibody
      • MRC 2 antibody
      • MRC2 antibody
      • MRC2_HUMAN antibody
      • UPAR-associated protein antibody
      • UPARAP antibody
      • Urokinase plasminogen activator receptor associated protein antibody
      • Urokinase receptor associated protein antibody
      • Urokinase receptor-associated protein antibody
      • Urokinase-type plasminogen activator receptor-associated protein antibody
      see all

    Anti-MRC2 antibody images

    • ab70132, at a 1/500 dilution, staining MRC2 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
    • ab70132, at a 1/50 dilution, staining MRC2 in paraffin embedded human brain tissue by Immunohistochemistry, in the absence or presence of the immunising peptide.
    • All lanes : Anti-MRC2 antibody (ab70132) at 1/500 dilution

      Lane 1 : 293 cell extracts
      Lane 2 : 293 cell extracts with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 167 kDa
      Observed band size : 167 kDa
    • ab70132 staining IMRC2 in tonsillar tissue by Flow Cytometry. Gating strategy : Viable leukocytes gated in FSC/SSC. The sample was incubated with the primary antibody (dilution 1/5 in PBS/0.5%BSA/2.5mM EDTA), for 30 minutes at 4°C. An FITC-conjugated goat polyclonal to Rabbit IgG (dilution 1/50) was used as secondary antibody.

      See Abreview

    • ab70132 staining MRC2 in Human pancreatic cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with Inhibitor CM for 4 minutes at 37°C; antigen retrieval was by heat mediation in Tris/Borate/EDTA buffer pH 8. Samples were incubated with primary antibody (1/50 ) for 32 minutes at 37°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    References for Anti-MRC2 antibody (ab70132)

    This product has been referenced in:
    • Gai X  et al. MRC2 expression correlates with TGFß1 and survival in hepatocellular carcinoma. Int J Mol Sci 15:15011-25 (2014). IHC-P ; Human . Read more (PubMed: 25162823) »
    • Ikenaga N  et al. Pancreatic cancer cells enhance the ability of collagen internalization during epithelial-mesenchymal transition. PLoS One 7:e40434 (2012). WB ; Human . Read more (PubMed: 22792318) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Pancreatic Cancer)
    Specification Pancreatic Cancer
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/Borate/EDTA buffer pH 8
    Permeabilization No
    Blocking step Inhibitor CM as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 19 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (pancreatic cancer cell line)
    Specification pancreatic cancer cell line
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.3 % triton X-100 in PBS
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 11 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Pancreatic Cancer)
    Loading amount 20 µg
    Specification Pancreatic Cancer
    Treatment 5ng/ml TGF-beta for 72 hours
    Gel Running Conditions Reduced Denaturing (4-12% polyacrylamide gel)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 05 2012

    Thank you for contacting Abcam about ab70132 (anti-human MRC2), Lot GR2002-1. We guarantee all our antibodies to be stable for: -6 months at 4C -5 years at -20C -10 years at -80C. Unfortunately we do not have anymore of that ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (tonsillar tissue)
    Specification tonsillar tissue
    Fixation None
    Permeabilization No
    Gating Strategy Viable leukocytes gated in FSC/SSC
    Username

    Abcam user community

    Verified customer

    Submitted Nov 10 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"