Anti-MRCL3 (phospho S18 + S19) antibody (ab194918)

Overview

  • Product name
    Anti-MRCL3 (phospho S18 + S19) antibody
    See all MRCL3 primary antibodies
  • Description
    Rabbit Polyclonal to MRCL3 (phospho S18 + S19)
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MRCL3 (phospho S19). The exact sequence is proprietary. A phospho specific peptide corresponding to residues surrounding S19 of Human MRCL3.
    Database link: P19105

  • Positive control
    • Starved 293 cell extracts; Human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 20 kDa.

Target

  • Function
    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • Sequence similarities
    Contains 3 EF-hand domains.
  • Post-translational
    modifications
    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
  • Information by UniProt
  • Database links
  • Alternative names
    • ML12A_HUMAN antibody
    • MLC 2B antibody
    • MLC-2B antibody
    • MLCB antibody
    • MYL12A antibody
    • Myosin regulatory light chain 12A antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2, nonsarcomeric antibody
    • Myosin regulatory light chain MRCL3 antibody
    • Myosin regulatory light chain MRLC3 antibody
    • Myosin RLC antibody
    • Myosin, light polypeptide, regulatory, non sarcomeric (20kD) antibody
    • nonsarcomeric antibody
    • RLC antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling MRCL3 (phospho S18) with ab194918 at 1/50 dilution.

  • All lanes : Anti-MRCL3 (phospho S18 + S19) antibody (ab194918) at 1/500 dilution

    Lane 1 : Untreated 293 cell extracts
    Lane 2 : Starvation treated 293 cell extracts


    Predicted band size : 20 kDa

References

ab194918 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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