Recombinant
RabMAb

Anti-MRCL3 (phospho S18 + T17) antibody [EPR1457] (ab126739)

Overview

  • Product name
    Anti-MRCL3 (phospho S18 + T17) antibody [EPR1457]
    See all MRCL3 primary antibodies
  • Description
    Rabbit monoclonal [EPR1457] to MRCL3 (phospho S18 + T17)
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to amino acids residues Threonine 17 and Serine 18 of Human MRLC3 (P19105).

  • Positive control
    • NIH/3T3 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR1457
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab126739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
    • Sequence similarities
      Contains 3 EF-hand domains.
    • Post-translational
      modifications
      Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
    • Information by UniProt
    • Database links
    • Alternative names
      • ML12A_HUMAN antibody
      • MLC 2B antibody
      • MLC-2B antibody
      • MLCB antibody
      • MYL12A antibody
      • Myosin regulatory light chain 12A antibody
      • Myosin regulatory light chain 2 antibody
      • Myosin regulatory light chain 2, nonsarcomeric antibody
      • Myosin regulatory light chain MRCL3 antibody
      • Myosin regulatory light chain MRLC3 antibody
      • Myosin RLC antibody
      • Myosin, light polypeptide, regulatory, non sarcomeric (20kD) antibody
      • nonsarcomeric antibody
      • RLC antibody
      see all

    Images

    • All lanes : Anti-MRCL3 (phospho S18 + T17) antibody [EPR1457] (ab126739) at 1/1000 dilution

      Lane 1 : NIH/3T3 cell lysate
      Lane 2 : NIH/3T3 cell lysate + Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 20 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)

    References

    ab126739 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab126739.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up