Overview

  • Product name
  • Description
    Rabbit polyclonal to Mre11
  • Tested applications
    Suitable for: IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human Mre11.

    (Peptide available as ab33124.)

  • Positive control
    • Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate; A431 (Human epithelial carcinoma cell line) Whole Cell Lysate;

Properties

Applications

Our Abpromise guarantee covers the use of ab33125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/200.

Target

  • Function
    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • Involvement in disease
    Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
  • Sequence similarities
    Belongs to the MRE11/RAD32 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • AT like disease antibody
    • Ataxia telangiectasia disorder like antibody
    • ATLD antibody
    • DNA recombination and repair protein antibody
    • Double strand break repair protein MRE11A antibody
    • Double-strand break repair protein MRE11A antibody
    • endo/exonuclease Mre11 antibody
    • HNGS1 antibody
    • meiotic recombination (S. cerevisiae) 11 homolog A antibody
    • Meiotic recombination 11 homolog 1 antibody
    • meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • Meiotic recombination 11 homolog A antibody
    • MmMRE11A antibody
    • Mre 11 antibody
    • MRE 11a antibody
    • MRE 11b antibody
    • MRE11 homolog 1 antibody
    • MRE11 homolog A antibody
    • MRE11 homolog double strand break repair nuclease antibody
    • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • MRE11 meiotic recombination 11 homolog A antibody
    • MRE11_HUMAN antibody
    • MRE11A antibody
    • MRE11b antibody
    • OTTHUMP00000236830 antibody
    • OTTHUMP00000236831 antibody
    • OTTHUMP00000236832 antibody
    • OTTHUMP00000236833 antibody
    see all

Images

  • All lanes : Anti-Mre11 antibody (ab33125) at 1 µg/ml

    Lane 1 : Jurkat whole cell lysate (ab7899)
    Lane 2 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 81 kDa
    Observed band size : 81 kDa
    Additional bands at : 49 kDa. We are unsure as to the identity of these extra bands.
  • Mre11 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to Mre11 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33125.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 81kDa: Mre11
  • ab33125 (1/200) stainning Mre11 in assynchronous HeLa cells (green). Cells were fixed using paraformaldehyde and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.

    See Abreview

References

This product has been referenced in:
  • Mariggiò G  et al. Kaposi Sarcoma Herpesvirus (KSHV) Latency-Associated Nuclear Antigen (LANA) recruits components of the MRN (Mre11-Rad50-NBS1) repair complex to modulate an innate immune signaling pathway and viral latency. PLoS Pathog 13:e1006335 (2017). WB ; Human . Read more (PubMed: 28430817) »
  • Chang L  et al. Targeting Rad50 sensitizes human nasopharyngeal carcinoma cells to radiotherapy. BMC Cancer 16:190 (2016). WB, IP, ICC/IF ; Human . Read more (PubMed: 26951044) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Specification
Lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Mar 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (LCLs)
Loading amount
50 µg
Specification
LCLs
Gel Running Conditions
Reduced Denaturing (6%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Ed Miller

Verified customer

Submitted Apr 02 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5%TritonX100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 29 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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