The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
Use at an assay dependent concentration.
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
Involvement in disease
Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
Belongs to the MRE11/RAD32 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
Mre11 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to Mre11 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33125. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Band: 81kDa: Mre11
Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody (ab33125)This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab33125 (1/200) stainning Mre11 in assynchronous HeLa cells (green). Cells were fixed using paraformaldehyde and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.
Mariggiò G et al. Kaposi Sarcoma Herpesvirus (KSHV) Latency-Associated Nuclear Antigen (LANA) recruits components of the MRN (Mre11-Rad50-NBS1) repair complex to modulate an innate immune signaling pathway and viral latency. PLoS Pathog13:e1006335 (2017).
Read more (PubMed: 28430817) »
Chang L et al. Targeting Rad50 sensitizes human nasopharyngeal carcinoma cells to radiotherapy. BMC Cancer16:190 (2016).
WB, IP, ICC/IF
Read more (PubMed: 26951044) »