Anti-Mre11 (phospho S264) antibody (ab74148)

Overview

  • Product nameAnti-Mre11 (phospho S264) antibody
    See all Mre11 primary antibodies
  • Description
    Rabbit polyclonal to Mre11 (phospho S264)
  • SpecificityThis antibody only detects Mre11 when phosphorylated at serine 264.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human Mre11 around the phosphorylation site of serine 264 (Y-I-SP-Q-P).

  • Positive control
    • 3T3 extract (treated with forskolin at 40nM for 30mins)

Properties

Applications

Our Abpromise guarantee covers the use of ab74148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
ELISA 1/5000.

Target

  • FunctionComponent of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • Involvement in diseaseDefects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
  • Sequence similaritiesBelongs to the MRE11/RAD32 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • AT like disease antibody
    • Ataxia telangiectasia disorder like antibody
    • ATLD antibody
    • DNA recombination and repair protein antibody
    • Double strand break repair protein MRE11A antibody
    • Double-strand break repair protein MRE11A antibody
    • endo/exonuclease Mre11 antibody
    • HNGS1 antibody
    • meiotic recombination (S. cerevisiae) 11 homolog A antibody
    • Meiotic recombination 11 homolog 1 antibody
    • meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • Meiotic recombination 11 homolog A antibody
    • MmMRE11A antibody
    • Mre 11 antibody
    • MRE 11a antibody
    • MRE 11b antibody
    • MRE11 homolog 1 antibody
    • MRE11 homolog A antibody
    • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • MRE11 meiotic recombination 11 homolog A antibody
    • MRE11_HUMAN antibody
    • MRE11A antibody
    • MRE11b antibody
    • OTTHUMP00000236830 antibody
    • OTTHUMP00000236831 antibody
    • OTTHUMP00000236832 antibody
    • OTTHUMP00000236833 antibody
    see all

Anti-Mre11 (phospho S264) antibody images

  • All lanes : Anti-Mre11 (phospho S264) antibody (ab74148) at 1/500 dilution

    Lane 1 : 3T3 extract (treated with forskolin at 40nM for 30mins)
    Lane 2 : 3T3 extract (treated with forskolin at 40nM for 30mins) with phosphopeptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 81 kDa
    Observed band size : 81 kDa

References for Anti-Mre11 (phospho S264) antibody (ab74148)

ab74148 has not yet been referenced specifically in any publications.

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