Anti-Mre11 (phospho S678) antibody (ab77631)

Overview

  • Product nameAnti-Mre11 (phospho S678) antibody
    See all Mre11 primary antibodies
  • Description
    Rabbit polyclonal to Mre11 (phospho S678)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Mre11, phosphorylated at S678.

    (Peptide available as ab112241.)

  • Positive control
    • This antibody gave a positive signal in Jurkat Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
    • Involvement in diseaseDefects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
    • Sequence similaritiesBelongs to the MRE11/RAD32 family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
    • Information by UniProt
    • Database links
    • Alternative names
      • AT like disease antibody
      • Ataxia telangiectasia disorder like antibody
      • ATLD antibody
      • DNA recombination and repair protein antibody
      • Double strand break repair protein MRE11A antibody
      • Double-strand break repair protein MRE11A antibody
      • endo/exonuclease Mre11 antibody
      • HNGS1 antibody
      • meiotic recombination (S. cerevisiae) 11 homolog A antibody
      • Meiotic recombination 11 homolog 1 antibody
      • meiotic recombination 11 homolog A (S. cerevisiae) antibody
      • Meiotic recombination 11 homolog A antibody
      • MmMRE11A antibody
      • Mre 11 antibody
      • MRE 11a antibody
      • MRE 11b antibody
      • MRE11 homolog 1 antibody
      • MRE11 homolog A antibody
      • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
      • MRE11 meiotic recombination 11 homolog A antibody
      • MRE11_HUMAN antibody
      • MRE11A antibody
      • MRE11b antibody
      • OTTHUMP00000236830 antibody
      • OTTHUMP00000236831 antibody
      • OTTHUMP00000236832 antibody
      • OTTHUMP00000236833 antibody
      see all

    Anti-Mre11 (phospho S678) antibody images

    • All lanes : Anti-Mre11 (phospho S678) antibody (ab77631) at 1 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human Mre11 (phospho S678) peptide (ab112241) at 1 µg/ml
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human Mre11 peptide (ab116269) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 81 kDa
      Observed band size : 85 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 140 kDa,50 kDa,70 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 20 minutes

    References for Anti-Mre11 (phospho S678) antibody (ab77631)

    ab77631 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"