This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-MRF antibody - Oligodendrocyte Marker (ab85464)


  • Product name
    Anti-MRF antibody - Oligodendrocyte Marker
    See all MRF primary antibodies
  • Description
    Rabbit polyclonal to MRF - Oligodendrocyte Marker

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1100 to the C-terminus of Human MRF.

  • Positive control
    • This antibody gave a positive signal in the following Mouse tissue lysates: Brain day 0; Embryonic brain E14; Embryonic brain E16.



Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).


  • Function
    Transcription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes.
  • Tissue specificity
    Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina.
  • Sequence similarities
    Belongs to the MRF family.
    Contains 1 NDT80 DNA-binding domain.
  • Domain
    A transmembrane region is predicted by sequence analysis tools. However, the protein is nuclear and probably acts as a transcription factor. It is therefore unclear whether the predicted transmembrane region is a hydrophobic region that does not insert into membranes or whether it is a real transmembrane region that may be cleaved when acting as transcription regulator.
  • Cellular localization
    Membrane. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C11orf9 antibody
    • Chromosome 11 open reading frame 9 antibody
    • GM1804 antibody
    • GM98 antibody
    • KIAA0954 antibody
    • MGC10781 antibody
    • MRF antibody
    • MRF_HUMAN antibody
    • Myelin gene regulatory factor antibody
    • Ndt80 antibody
    see all


This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-MRF antibody - Oligodendrocyte Marker (ab85464) at 1/250 dilution

    Lane 1 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 2 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 3 : E16 Ms Embryo Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 123 kDa
    Observed band size: 123 kDa
    Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 4 minutes

    Further characterization on this product is underway. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab85464 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.


ab85464 has not yet been referenced specifically in any publications.

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