Overview

  • Product nameAnti-MRG15 antibody
    See all MRG15 primary antibodies
  • Description
    Rabbit polyclonal to MRG15
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (KDKQVKYFIH YSGWNKNWDE WVPESRVLKY VDTNLQKQRE LQKANQEQYA) of Human MRG15 (NP_006782).

  • Positive control
    • Placenta lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionComponent of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. Required for homologous recombination repair (HRR) and resistance to mitomycin C (MMC). Involved in the localization of PALB2, BRCA2 and RAD51, but not BRCA1, to DNA-damage foci.
  • Sequence similaritiesBelongs to the MRG family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CG6363 antibody
    • Eaf3 antibody
    • Esa1p associated factor 3 homolog antibody
    • FWP006 antibody
    • HsT17725 antibody
    • MEAF3 antibody
    • MO4L1_HUMAN antibody
    • MORF related gene on chromosome 15 antibody
    • MORF-related gene 15 protein antibody
    • Morf4l1 antibody
    • MORFRG15 antibody
    • Mortality factor 4 like 1 antibody
    • Mortality factor 4 like protein 1 antibody
    • Mortality factor 4-like protein 1 antibody
    • MRG15 antibody
    • Protein MSL3-1 antibody
    • S863-6 antibody
    • Transcription factor-like protein MRG15 antibody
    see all

Anti-MRG15 antibody images



  • Predicted band size : 41 kDa
    Western blot analysis of Human adult placenta tissue lysate labeling MRG15 with ab84520 at 1.0µg/ml.
  • Anti-MRG15 antibody (ab84520) at 1 µg/ml (5% skim milk / PBS buffer) + placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%

References for Anti-MRG15 antibody (ab84520)

ab84520 has not yet been referenced specifically in any publications.

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