Anti-MRGX1 antibody (ab77519)
Key features and details
- Rabbit polyclonal to MRGX1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MRGX1 antibody -
Description
Rabbit polyclonal to MRGX1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from internal sequence of human GPCR MRGX1.
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Positive control
- Extracts from MCF-7 cells. HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22)(PubMed:26582731). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells. G(alpha)q proteins are involved in the calcium-signaling pathway. Activated by the antimalarial drug, chloroquine. May mediate chloroquine-induced itch, in a histamine-independent manner. -
Tissue specificity
Uniquely localized in a subset of small dorsal root and trigeminal sensory neurons. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Mas subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 259249 Human
- Omim: 607227 Human
- SwissProt: Q96LB2 Human
- Unigene: 711459 Human
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Alternative names
- GPCR antibody
- MAS related gene MRGX1 antibody
- MAS related GPR member X1 antibody
see all
Images
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All lanes : Anti-MRGX1 antibody (ab77519) at 1/500 dilution
Lane 1 : Extracts from MCF-7 cells
Lane 2 : Extracts from MCF-7 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa -
Immunofluorescence analysis of GPCR MRGX1 in HepG2 cells using ab77519, at 1/500 dilution, in the absence or presence of immunising peptide
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab77519 has not yet been referenced specifically in any publications.