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Rabbit polyclonal to MRGX1
Synthetic peptide derived from internal sequence of human GPCR MRGX1.
Extracts from MCF-7 cells. HepG2 cells.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 36 kDa.
1/500 - 1/1000.
MRGX1 is an opiod receptor that has been reported exclusively in dorsal root ganglion. MRGX1 is probably involved in the function of nociceptive neurons and may regulate nociceptor function and/or development, including the sensation or modulation of pain. It is potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells.
Cell Membrane; Multi-pass membrane protein.
MAS related gene MRGX1 antibody
MAS related GPR member X1 antibody
Western blot - Anti-MRGX1 antibody (ab77519)
All lanes : Anti-MRGX1 antibody (ab77519) at 1/500 dilution Lane 1 : Extracts from MCF-7 cells Lane 2 : Extracts from MCF-7 cells with immunising peptide at 5 µg Lysates/proteins at 5 µg per lane. Predicted band size: 36 kDa Observed band size: 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MRGX1 antibody (ab77519)
Immunofluorescence analysis of GPCR MRGX1 in HepG2 cells using ab77519, at 1/500 dilution, in the absence or presence of immunising peptide
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"