Overview

  • Product nameAnti-MRGX1 antibody
  • Description
    Rabbit polyclonal to MRGX1
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from internal sequence of human GPCR MRGX1.

  • Positive control
    • Extracts from MCF-7 cells. HepG2 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • RelevanceMRGX1 is an opiod receptor that has been reported exclusively in dorsal root ganglion. MRGX1 is probably involved in the function of nociceptive neurons and may regulate nociceptor function and/or development, including the sensation or modulation of pain. It is potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells.
  • Cellular localizationCell Membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • GPCR antibody
    • MAS related gene MRGX1 antibody
    • MAS related GPR member X1 antibody
    • MRGPRX1 antibody
    • MRGX1 antibody
    • SNSR4 antibody
    see all

Anti-MRGX1 antibody images

  • All lanes : Anti-MRGX1 antibody (ab77519) at 1/500 dilution

    Lane 1 : Extracts from MCF-7 cells
    Lane 2 : Extracts from MCF-7 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 36 kDa
    Observed band size : 36 kDa
  • Immunofluorescence analysis of GPCR MRGX1 in HepG2 cells using ab77519, at 1/500 dilution, in the absence or presence of immunising peptide

References for Anti-MRGX1 antibody (ab77519)

ab77519 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"