Overview

  • Product nameAnti-MRP1 antibody [MRPr1]
    See all MRP1 primary antibodies
  • Description
    Rat monoclonal [MRPr1] to MRP1
  • SpecificityThis antibody detects MRP 1. It does not cross-react with the human MDR 1 and MDR 3 P glycoprotein gene products.
  • Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Bacterial fusion protein of human MRP 1 containing a segment of 168 amino acids inthe amino-proximal half of the protein.

  • EpitopeInternal epitope.
  • Positive control
    • Cell Lines: Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Tissue section: Breast carcinoma

Applications

Our Abpromise guarantee covers the use of ab3368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr 1/20 - 1/50.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionMediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
  • Tissue specificityLung, testis and peripheral blood mononuclear cells.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 29 antibody
    • ABC29 antibody
    • ABCC 1 antibody
    • ABCC antibody
    • Abcc1 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
    • ATP binding cassette sub-family C member 1 antibody
    • ATP-binding cassette sub-family C member 1 antibody
    • DKFZp686N04233 antibody
    • DKFZp781G125 antibody
    • GS X antibody
    • GSX antibody
    • Leukotriene C(4) transporter antibody
    • LTC4 transporter antibody
    • MRP 1 antibody
    • MRP antibody
    • MRP1_HUMAN antibody
    • Multidrug resistance associated protein 1 antibody
    • Multidrug resistance protein antibody
    • Multidrug resistance-associated protein 1 antibody
    • Multiple drug resistance associated protein antibody
    • Multiple drug resistance protein 1 antibody
    see all

Anti-MRP1 antibody [MRPr1] images

  • ICC/IF image of ab3368 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3368,5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab3368 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3368, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-MRP1 antibody [MRPr1] (ab3368)

This product has been referenced in:
  • Hirata N  et al. Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation. Nat Commun 5:4806 (2014). Read more (PubMed: 25254944) »
  • Juuti-Uusitalo K  et al. Efflux protein expression in human stem cell-derived retinal pigment epithelial cells. PLoS One 7:e30089 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22272278) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - other (MCF7)
Loading amount 30 µg
Specification MCF7
Treatment drug or DMSO
Gel Running Conditions Reduced Non-Denaturing (Native) (lysates not heated and no b-mercapto or DTT added to loading buffer)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Apr 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Breast)
Specification Human Breast
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted Apr 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"