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Bacterial fusion protein of human MRP 2 containing the carboxy-terminal region (residues 1339-1541).
We received mixed feedback about rat species so we are removing this from tested species list for now. The immunogen is 84% homologous to human protein so theoretically it should cross react however considering the latest customer feedback we no longer guarantee this species.
Our Abpromise guarantee covers the use of ab3373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 18929567
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration. PubMed: 16687572|
|ICC||1/20 - 1/50.
Acetone fixed cytospin preparations.
|IHC-P||1/200. See Abreview. Abreview indicates that several methods of antigen retreival are suitable for this antibody, but heat mediated Ag retrieval in citrate buffer gave optimal results.|
Acetone fixation of frozen sample is recommended.
|WB||1/20 - 1/50. Predicted molecular weight: 174 kDa.
See Scheffer GL et al. 2000.
The samples MUST NOT be boiled before running the gel as multi-pass transmembrane proteins, like MRP2, aggregate severely at high temperatures, which increases as a direct function of temperature. We recommend heating the samples at 60-70C for 15-20 minutes or at 36C for 20 minutes as mentioned in PLoS Pathog 9:e1003244 (2013).