Overview

  • Product name
    Anti-MRP2 antibody [M2 III-6]
    See all MRP2 primary antibodies
  • Description
    Mouse monoclonal [M2 III-6] to MRP2
  • Specificity
    This antibody detects MRP 2. It does not cross-react with human MDR 1, MRP 1, MRP 3 or MRP 5 gene products.
  • Tested applications
    Suitable for: Flow Cyt, IP, ICC, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Bacterial fusion protein of human MRP 2 containing the carboxy-terminal region (residues 1339-1541).

  • Epitope
    This antibody reacts with an internal epitope of MRP 2.
  • General notes

    We received mixed feedback about rat species so we are removing this from tested species list for now. The immunogen is 84% homologous to human protein so theoretically it should cross react however considering the latest customer feedback we no longer guarantee this species.

Properties

Applications

Our Abpromise guarantee covers the use of ab3373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 18929567

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration. PubMed: 16687572
ICC 1/20 - 1/50.

Acetone fixed cytospin preparations.

IHC-P 1/200. See Abreview. Abreview indicates that several methods of antigen retreival are suitable for this antibody, but heat mediated Ag retrieval in citrate buffer gave optimal results.
IHC-Fr 1/20.

Acetone fixation of frozen sample is recommended.

WB 1/20 - 1/50. Predicted molecular weight: 174 kDa.

See Scheffer GL et al. 2000.

The samples MUST NOT be boiled before running the gel as multi-pass transmembrane proteins, like MRP2, aggregate severely at high temperatures, which increases as a direct function of temperature. We recommend heating the samples at 60-70C for 15-20 minutes or at 36C for 20 minutes as mentioned in PLoS Pathog 9:e1003244 (2013).
The protein is glycosylated at amino acid 7, 12 and 1011 so higher band size is expected.

Target

  • Function
    Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
  • Tissue specificity
    Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
  • Involvement in disease
    Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC30 antibody
    • abcC2 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
    • ATP binding cassette subfamily C member 2 antibody
    • ATP-binding cassette sub-family C member 2 antibody
    • Canalicular multidrug resistance protein antibody
    • Canalicular multispecific organic anion transporter 1 antibody
    • CMOAT antibody
    • CMOAT1 antibody
    • cMRP antibody
    • DJS antibody
    • KIAA1010 antibody
    • MRP 2 antibody
    • MRP2_HUMAN antibody
    • Multidrug resistance associated protein 2 antibody
    • Multidrug resistance-associated protein 2 antibody
    see all

Anti-MRP2 antibody [M2 III-6] images

  • Immunohistochemical analysis of Human liver tissue, staining MRP2 with ab3373 at 1/50 dilution.

References for Anti-MRP2 antibody [M2 III-6] (ab3373)

This product has been referenced in:
  • Bell CC  et al. Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease. Sci Rep 6:25187 (2016). IHC-Fr ; Human . Read more (PubMed: 27143246) »
  • Kwon MH  et al. Effects of poloxamer 407-induced hyperlipidemia on hepatic multidrug resistance protein 2 (Mrp2/Abcc2) and the pharmacokinetics of mycophenolic acid in rats. Biopharm Drug Dispos 37:352-65 (2016). Read more (PubMed: 27241939) »

See all 21 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
15 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jan 24 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Nov 17 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (WIF-B cells, the hepatoma-derived hybrid cell line)
Gel Running Conditions
Reduced Denaturing (6% SDS-PAGE)
Loading amount
30 µg
Specification
WIF-B cells, the hepatoma-derived hybrid cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 09 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2 cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
HepG2 cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Oct 14 2016


I can confirm that anti MRP2 antibody ab3373 does not cross react with other MRPs.

Thank you for your reply and for kindly providing the requested details.

I look forward to hearing from you with the results of the next experiment. i can recommend it would be beneficial to consider a reducing gel. This will ensure the prot...

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Thank you for taking the time to complete our questionnaire. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies.

I am sorry to confirm that because we carry over 90,000 products, it is regrettably not feasible fo...

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Any products that require an MSDS form will have a link on the product's online datasheet to the data. It will be a PDF document link labeled 'SDS'. Please see attached image for example of what to look for on the datasheet. If no 'SDS' link ...

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Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab241...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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