Overview

  • Product nameAnti-MRP2 antibody [M2III-5]
    See all MRP2 primary antibodies
  • Description
    Mouse monoclonal [M2III-5] to MRP2
  • SpecificityThis antibody does not cross-react with the human MDR1 P-gp, MRP1, MRP3, or MRP5 gene products.
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
    Does not react with: Mouse
  • Immunogen

    This antibody was raised against a fusion protein of the bacterial maltose binding protein and rat MRP2, containing the 202-amino acid C terminal end of the transporter protein.

  • Epitopeab15603 reacts with an internal epitope of MRP2; a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome.

Properties

Applications

Our Abpromise guarantee covers the use of ab15603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20 - 1/50. Predicted molecular weight: 185.4 kDa.
Flow Cyt Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC 1/20 - 1/50. Acetone fixed cytospin preparations.
IHC-P 1/20. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/20. Acetone fixed.

Target

  • FunctionMediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
  • Tissue specificityFound on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
  • Involvement in diseaseDefects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC30 antibody
    • abcC2 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
    • ATP binding cassette subfamily C member 2 antibody
    • ATP-binding cassette sub-family C member 2 antibody
    • Canalicular multidrug resistance protein antibody
    • Canalicular multispecific organic anion transporter 1 antibody
    • CMOAT antibody
    • CMOAT1 antibody
    • cMRP antibody
    • DJS antibody
    • KIAA1010 antibody
    • MRP 2 antibody
    • MRP2_HUMAN antibody
    • Multidrug resistance associated protein 2 antibody
    • Multidrug resistance-associated protein 2 antibody
    see all

References for Anti-MRP2 antibody [M2III-5] (ab15603)

This product has been referenced in:
  • Hardwick RN  et al. Molecular mechanism of altered ezetimibe disposition in nonalcoholic steatohepatitis. Drug Metab Dispos 40:450-60 (2012). WB ; Rat . Read more (PubMed: 22112382) »
  • Saeki J  et al. LPS-induced dissociation of multidrug resistance-associated protein 2 (Mrp2) and radixin is associated with Mrp2 selective internalization in rats. Biochem Pharmacol 81:178-84 (2011). WB, IHC-Fr ; Rat . Read more (PubMed: 20868650) »

See all 8 Publications for this product

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The note regarding mouse specificity is based on data from the following reference, which includes a description of the clone's development and characterization:

Cancer Res. 2000 Sep 15;60(18):5269-77....

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I have inquired with the laboratories of the polyclonal antibodies, whether the immunogen sequence is conserved with the pig sequence. This would then provid...

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Thank you for your feedback regarding this product. As a result of the information that you have sent to me and references sourced via “Pub Med” I have currently removed cross reactivity with from our product data base. I would like to thank you for th...

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