Overview

  • Product name
    Anti-MRP3 antibody [M3II-21]
    See all MRP3 primary antibodies
  • Description
    Mouse monoclonal [M3II-21] to MRP3
  • Specificity
    This antibody does not cross-react with MDR 1, MRP 1, MRP 2 or MRP 5 gene products.
  • Tested applications
    Suitable for: WB, ICC, IHC-Fr, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Bacterial fusion protein of human MRP 3 containing amino acids 830-949.

  • Epitope
    This antibody detects with an internal epitope of the membrane transport protein of MRP 3.
  • Positive control
    • Drug-sensitive parental cell lines and their multidrug-resistant derivatives

Properties

Applications

Our Abpromise guarantee covers the use of ab3376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20 - 1/50.
ICC 1/20 - 1/50. Fix with acetone or formaldehyde.
IHC-Fr 1/20 - 1/40. Fix with acetone. Use amplification system such as the ABC approach.
Flow Cyt Use at an assay dependent concentration. PubMed: 18630504
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes.
    • Tissue specificity
      Mainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney.
    • Sequence similarities
      Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABC 31 antibody
      • ABC31 antibody
      • ABCC 3 antibody
      • ABCC3 antibody
      • ATP binding cassette sub family C (CFTR/MRP) member 3 antibody
      • ATP binding cassette sub family C member 3 antibody
      • ATP-binding cassette sub-family C member 3 antibody
      • Canalicular multispecific organic anion transporter 2 antibody
      • Canicular multispecific organic anion transporter antibody
      • CMOAT 2 antibody
      • CMOAT2 antibody
      • EST90757 antibody
      • MLP 2 antibody
      • MLP2 antibody
      • MOAT D antibody
      • MOAT-D antibody
      • MOATD antibody
      • MRP 3 antibody
      • MRP3_HUMAN antibody
      • Multi specific organic anion transporter D antibody
      • Multi-specific organic anion transporter D antibody
      • Multidrug resistance associated protein 3 antibody
      • Multidrug resistance associated protein antibody
      • Multidrug resistance-associated protein 3 antibody
      • Multispecific organic anion tranporter D antibody
      see all

    References

    This product has been referenced in:
    • Chang HW  et al. Characterization of a transgenic mouse model exhibiting spontaneous lung adenocarcinomas with a metastatic phenotype. PLoS One 12:e0175586 (2017). IHC-P ; Mouse . Read more (PubMed: 28419107) »
    • Wittgen HG  et al. Cannabinoid type 1 receptor antagonists modulate transport activity of multidrug resistance-associated proteins MRP1, MRP2, MRP3, and MRP4. Drug Metab Dispos 39:1294-302 (2011). Human . Read more (PubMed: 21511945) »

    See all 6 Publications for this product

    Customer reviews and Q&As

    Thank you for your e-mail. I think what you are observing is the protein in its heavily glycosylated form. I had similar bands when I work on the TrkB receptor which is also very glycosylated. I am forwarding your images to the laboratory for confirm...

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    Many thanks for your detailed protocol which helped me understand your problem very rapidly. We have been informed by the laboratory that tested the antibody that it has been tested at a dilution of 1/20-1/50. This is surprisingly low (and can be du...

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    The concentration of Abcam batch 93034 is 250 ug/ml. If you have any additional questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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