Overview

  • Product nameAnti-MRP3 antibody [M3II-9]
    See all MRP3 primary antibodies
  • Description
    Mouse monoclonal [M3II-9] to MRP3
  • SpecificityThis antibody does not cross react with MDR1, MRP1, MRP2 or MRP5 gene products
  • Tested applicationsSuitable for: Flow Cyt, ICC, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein:

    SEMGPYPALL QRNGSFANFL CNYAPDEDQG HLEDSWTALE GAEDKEALLI EDTLSNHTD LTDNDPVTYV VQKQFMRQLS ALSSDGEGQG RPVPRRHLGPS EKVQVTEAKA DGALTQEEKA

    , corresponding to amino acids 830-949 of Human MRP3.

  • EpitopeThis antibody detects with an internal epitope of the membrane transport protein of MRP3
  • Positive control
    • Cell lines: Drug sensitive parental cell lines and their multidrug resistant derivatives
  • General notes

    The rat species was added as a result of positive Abreviews and publication PMID 22112382. In response to few recent complaints, we have decided to remove this species and no longer guarantee it. Please contact technical@abcam.com for any inquiry.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC 1/20 - 1/50.

Acetone or formaldehyde fixation is recommended for cytospin preparations.

IHC-Fr 1/20 - 1/40.

Acetone fixation of sections is recommended.

WB 1/20 - 1/50. Detects a band of approximately 190 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 21878559

Target

  • FunctionMay act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes.
  • Tissue specificityMainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 31 antibody
    • ABC31 antibody
    • ABCC 3 antibody
    • ABCC3 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 3 antibody
    • ATP binding cassette sub family C member 3 antibody
    • ATP-binding cassette sub-family C member 3 antibody
    • Canalicular multispecific organic anion transporter 2 antibody
    • Canicular multispecific organic anion transporter antibody
    • CMOAT 2 antibody
    • CMOAT2 antibody
    • EST90757 antibody
    • MLP 2 antibody
    • MLP2 antibody
    • MOAT D antibody
    • MOAT-D antibody
    • MOATD antibody
    • MRP 3 antibody
    • MRP3_HUMAN antibody
    • Multi specific organic anion transporter D antibody
    • Multi-specific organic anion transporter D antibody
    • Multidrug resistance associated protein 3 antibody
    • Multidrug resistance associated protein antibody
    • Multidrug resistance-associated protein 3 antibody
    • Multispecific organic anion tranporter D antibody
    see all

Anti-MRP3 antibody [M3II-9] images


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 minutes

     HepG2 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of clotrimazole (ab120744) in DMSO. Increased expression of MRP3 (ab3375) correlates with an increase in clotrimazole concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3375 at 1/50 dilution and ab9484 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.

References for Anti-MRP3 antibody [M3II-9] (ab3375)

This product has been referenced in:
  • Hardwick RN  et al. Molecular mechanism of altered ezetimibe disposition in nonalcoholic steatohepatitis. Drug Metab Dispos 40:450-60 (2012). WB ; Rat . Read more (PubMed: 22112382) »
  • Hardwick RN  et al. Variations in ATP-binding cassette transporter regulation during the progression of human nonalcoholic fatty liver disease. Drug Metab Dispos 39:2395-402 (2011). WB, IHC-P ; Human . Read more (PubMed: 21878559) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab241...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Liver)
Loading amount 80 µg
Specification Liver
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Aug 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver homogenate)
Loading amount 25 µg
Specification Liver homogenate
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 17 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (lung carcinoma)
Loading amount 25 µg
Specification lung carcinoma
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 19 2009

Thank you for your enquiry and for your patience. Unfortunately, none of these antibodies are available in a conjugated form but we do have a wide range of secondary antibodies to choose from (including ones conjugated to FITC and PE). At the bottom of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"