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Rabbit polyclonal to MRP4
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MRP4.
(Peptide available as
ab32550 gave a positive signal in the following Human Tissue Lysates: Testis, Spleen, Thymus, Kidney and Prostate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 150 kDa).
May be an organic anion pump relevant to cellular detoxification.
Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.
Information by UniProt
ABCC 4 antibody
ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
Western blot - Anti-MRP4 antibody (ab32550)
All lanes :
Anti-MRP4 antibody (ab32550) at 1 µg/ml
Lane 1 :
Prostate (Human) Tissue Lysate
Lane 2 :
Human kidney tissue lysate - total protein (
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
230 kDa (
why is the actual band size different from the predicted?
Additional bands at:
32 kDa, 42 kDa, 53 kDa. We are unsure as to the identity of these extra bands.
MRP4 has a predicted molecular weight of 150 kDa; however it has a number of potential transmembrane regions, glycosylation and phosphotylation sites which may affect the migration of the protein (SwissProt data). Abcam currently sells another antibody (
) which detects a band of similar molecular weight in Hela lysate.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"