Overview

  • Product name
  • Description
    Mouse monoclonal to MRP4
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-110 of Human MRP4

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab56675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 150 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    May be an organic anion pump relevant to cellular detoxification.
  • Tissue specificity
    Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABCC 4 antibody
    • ABCC4 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
    • ATP binding cassette sub family C member 4 antibody
    • ATP-binding cassette sub-family C member 4 antibody
    • bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4) antibody
    • bA464I2.1 antibody
    • Canalicular multispecific organic anion transporter antibody
    • Canalicular multispecific organic anion transporter ABC superfamily antibody
    • EST170205 antibody
    • MOAT B antibody
    • MOAT-B antibody
    • MOATB antibody
    • MRP 4 antibody
    • MRP/cMOAT related ABC transporter antibody
    • MRP/cMOAT-related ABC transporter antibody
    • MRP4_HUMAN antibody
    • Multi specific organic anion transporter B antibody
    • Multi-specific organic anion transporter B antibody
    • Multidrug resistance associated protein 4 antibody
    • Multidrug resistance-associated protein 4 antibody
    • OTTHUMP00000018560 antibody
    see all

Images



  • Predicted band size : 150 kDa
    MRP4 antibody (ab56675) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab56675 stained HepG2 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56675, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Overlay histogram showing PC3 cells stained with ab56675 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56675, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

This product has been referenced in:
  • Meisenberg C  et al. Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res 45:1159-1176 (2017). WB ; Human . Read more (PubMed: 28180300) »
  • Wang Z  et al. Suppression of c-Myc is involved in multi-walled carbon nanotubes' down-regulation of ATP-binding cassette transporters in human colon adenocarcinoma cells. Toxicol Appl Pharmacol 282:42-51 (2015). Read more (PubMed: 25461681) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for contacting us and reporting the problems you have been experiencing in using theanti-MRP4 antibody (ab56675) with Western blotting of sheep samples.


As discussed over the phone, this antibody has as yet not been tested wit...

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