Anti-MRP4 antibody [M4I-10] (ab15602)
Key features and details
- Rat monoclonal [M4I-10] to MRP4
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-MRP4 antibody [M4I-10]
See all MRP4 primary antibodies -
Description
Rat monoclonal [M4I-10] to MRP4 -
Host species
Rat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Fusion protein corresponding to Human MRP4 aa 350-450. Fusion protein containing the E. coli maltose binding protein and a fragment of the human MRP4 protein corresponding to amino acids 372-431.
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Positive control
- Kidney tissue This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
M4I-10 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/2000. Predicted molecular weight: 159 kDa.
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000. Predicted molecular weight: 159 kDa. |
Target
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Function
May be an organic anion pump relevant to cellular detoxification. -
Tissue specificity
Widely expressed, with particularly high levels in prostate, but is barely detectable in liver. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10257 Human
- Entrez Gene: 239273 Mouse
- Omim: 605250 Human
- SwissProt: O15439 Human
- Unigene: 508423 Human
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Alternative names
- ABCC 4 antibody
- ABCC4 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
see all
Images
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All lanes : Anti-MRP4 antibody [M4I-10] (ab15602) at 20 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ABCC4 (MRP4) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 159 kDaLanes 1 - 2: Merged signal (red and green). Green - ab15602 observed at 200-250 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab15602 was shown to specifically react with MRP4 in wild-type HAP1 cells as signal was lost in ABCC4 (MRP4) knockout cells. Wild-type and ABCC4 (MRP4) knockout samples were subjected to SDS-PAGE. Ab15602 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 20 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
HC image of MRP4 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15602, 10µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody (ab253031) was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab15602 has been referenced in 36 publications.
- Grange RMH et al. Multidrug resistance proteins preferentially regulate natriuretic peptide-driven cGMP signalling in the heart and vasculature. Br J Pharmacol 179:2443-2459 (2022). PubMed: 34131904
- Tsurudome Y et al. Decreased ZO1 expression causes loss of time-dependent tight junction function in the liver of ob/ob mice. Mol Biol Rep 49:11881-11890 (2022). PubMed: 36224445
- Nguyen JP et al. Modulation of cAMP metabolism for CFTR potentiation in human airway epithelial cells. Sci Rep 11:904 (2021). PubMed: 33441643
- Wu DM et al. Nrf2 overexpression rescues the RPE in mouse models of retinitis pigmentosa. JCI Insight 6:N/A (2021). PubMed: 33491671
- Zhou Y et al. Drug Elimination Alteration in Acute Lymphoblastic Leukemia Mediated by Renal Transporters and Glomerular Filtration. Pharm Res 37:158 (2020). PubMed: 32743772