Overview

  • Product nameAnti-MRP4 antibody [M4I-10]
    See all MRP4 primary antibodies
  • Description
    Rat monoclonal [M4I-10] to MRP4
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WB, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    This antibody was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the human MRP4 protein corresponding to amino acids 372-431.

  • Positive control
    • Kidney tissue This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab15602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 22272278PFA fixed cells
WB 1/20 - 1/50. Predicted molecular weight: 159 kDa.
IHC-Fr 1/20. Fix with acetone.
ICC 1/20 - 1/50. (acetone fixed cytospin preparations)

Target

  • FunctionMay be an organic anion pump relevant to cellular detoxification.
  • Tissue specificityWidely expressed, with particularly high levels in prostate, but is barely detectable in liver.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABCC 4 antibody
    • ABCC4 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
    • ATP binding cassette sub family C member 4 antibody
    • ATP-binding cassette sub-family C member 4 antibody
    • bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4) antibody
    • bA464I2.1 antibody
    • Canalicular multispecific organic anion transporter antibody
    • Canalicular multispecific organic anion transporter ABC superfamily antibody
    • EST170205 antibody
    • MOAT B antibody
    • MOAT-B antibody
    • MOATB antibody
    • MRP 4 antibody
    • MRP/cMOAT related ABC transporter antibody
    • MRP/cMOAT-related ABC transporter antibody
    • MRP4_HUMAN antibody
    • Multi specific organic anion transporter B antibody
    • Multi-specific organic anion transporter B antibody
    • Multidrug resistance associated protein 4 antibody
    • Multidrug resistance-associated protein 4 antibody
    • OTTHUMP00000018560 antibody
    see all

Anti-MRP4 antibody [M4I-10] images

  • HC image of MRP4 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15602, 10µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab15602 staining MRP4 (green) in early cobblestone Human ESC-RPEs by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde and blocked with 3% BSA in PBS for 1 hour at room temperature; antigen retrieval was by 0.1% Triton X-100/PBS. Samples were incubated with primary antibody (1/100 in 0.5% BSA-PBS) for 2 hours at room temperature. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody.

References for Anti-MRP4 antibody [M4I-10] (ab15602)

This product has been referenced in:
  • Rohrer PR  et al. Is nuclear factor erythroid 2-related factor 2 responsible for sex differences in susceptibility to acetaminophen-induced hepatotoxicity in mice? Drug Metab Dispos 42:1663-74 (2014). Read more (PubMed: 25092713) »
  • Steinberg F  et al. A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Nat Cell Biol 15:461-71 (2013). Human . Read more (PubMed: 23563491) »

See all 12 Publications for this product

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In order to find the secondary antibody that best fits your necessities; I would like you to provide us with further information, such as in which application are you testing the antibody or the host species for the secondary.
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I look forward to hearing from you with the results of the next experiment. i can recommend it would be beneficial to consider a reducing gel. This will ensure the prot...

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I am sorry to confirm that because we carry over 90,000 products, it is regrettably not feasible fo...

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Thank you for your enquiry. The publication found at the following link contains images of Western blots for both of these antibodies: http://mcb.asm.org/cgi/content/full/24/17/7612?view=long&pmid=15314169 I hope this helps. Please contact us ...

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Thank you for your enquiry. I am sorry for the delay to your enquiry; I have been in touch with the source of this antibody and a response has not been forthcoming. For the testing of antibodies ab15598 and ab15602 against MRP4 I have been informed...

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Thank you for contacting us for technical support with ab15602. I am currently trying to find out the recommended positive control for this antibody and as soon as I have this information I will let you know. The antibody is supplied as tissue cult...

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Thank you for your enquiry regarding ab15602. This antibody has not been tested in rat (only human and mouse) but we would not advise to use it in rat in IHC as the antibody has been raised in rat and hence the secondary antibody anti-rat is very like...

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