Overview

  • Product nameAnti-MRP5 antibody [M5II-54]
    See all MRP5 primary antibodies
  • Description
    Rat monoclonal [M5II-54] to MRP5
  • Specificityab24107 does not cross-react with the human MDR1, MRP1, MRP2 or MRP3 gene products
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Fusion protein, corresponding to amino acids 722-910 of Human MRP5.

  • Epitopeab24107 reacts with an internal epitope of MRP5. ab24107 does not cross-react with the human MDR1, MRP1, MRP2 or MRP3 gene products
  • General notes


    ab24107 has potential value for detection of MRP5-mediated drug-resistance in human tumor samples

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.7% BSA, Tissue culture supernatant (serum free)
  • PurityTissue culture supernatant
  • Purification notesAntibody containing supernatant has been concentrated and filtered through a 0.22 micron filter and is serum free.
  • Primary antibody notesab24107 has potential value for detection of MRP5-mediated drug-resistance in human tumor samples
  • ClonalityMonoclonal
  • Clone numberM5II-54
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/20.
Flow Cyt Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/20 - 1/50.
IP Use at an assay dependent concentration. PubMed: 19323627
IHC-P Use at an assay dependent concentration. PubMed: 19323627
WB 1/20 - 1/50. Predicted molecular weight: 161 kDa.

Target

  • FunctionActs as a multispecific organic anion pump which can transport nucleotide analogs.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 33 antibody
    • ABC33 antibody
    • ABCC 5 antibody
    • Abcc5 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 5 antibody
    • ATP binding cassette sub family C member 5 antibody
    • ATP-binding cassette sub-family C member 5 antibody
    • Canalicular multispecific organic anion transporter C antibody
    • DKFZp686C1782 antibody
    • EST277145 antibody
    • MOAT C antibody
    • MOAT-C antibody
    • MOATC antibody
    • MRP 5 antibody
    • MRP5_HUMAN antibody
    • Multi specific organic anion tranporter C antibody
    • Multi-specific organic anion transporter C antibody
    • Multidrug resistance associated protein 5 antibody
    • Multidrug resistance-associated protein 5 antibody
    • pABC 11 antibody
    • pABC11 antibody
    • SMRP antibody
    see all

Anti-MRP5 antibody [M5II-54] images

  • Overlay histogram showing Y79 cells stained with ab24107 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24107, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H&L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Y79 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-MRP5 antibody [M5II-54] (ab24107)

This product has been referenced in:
  • Karla PK  et al. Expression of multidrug resistance associated protein 5 (MRP5) on cornea and its role in drug efflux. J Ocul Pharmacol Ther 25:121-32 (2009). IP, IHC-P ; Human . Read more (PubMed: 19323627) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. As you have mentioned DAPI stains the nuclei so it helps in Immunocytochemistry to see whether the target staining observed in the images is definitely because of cells not the background staining (binding of antibody to de...

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Thank you for contacting us. The practice of antigen retrieval is widely employed with paraffin-embedded tissue sections only.It is less often applied to cells; even in our own laboratory we generally test antibodiesby Immunohistochemistry on tissue se...

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Thank you for your reply and the additional information. I would like to offer a couple troubleshooting tips that might help with improving the results: 1) Using more primary antibody (maybe a 1/20 dilution) might help. 2) Trying another anigen ...

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Thank you for contacting us. I am sorry to hear that this antibody is not working well. The antibody has been tested only on human samples, but not on mouse and rat. Mouse and rat are only predicted species for this antibody and we do not have any data...

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Thank you for your enquiry. The optimal dilution for FACS has not been determined for this antibody. As a general recommendation, we would suggest using 1-10ug/ml per 10^6 cells. If you have any additional questions, please do not hesitate to c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"