The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
Use 0.5µg for 106 cells. ab91368-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
Tissue specificityExpressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses.
Sequence similaritiesBelongs to the S-100 family. Contains 2 EF-hand domains.
Cellular localizationSecreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
Cell Source: Human Peripheral Blood Leukocytes labeled with ab112213 at a concentration of 0.5µg/106 Cells.
Percentage of cells stained above control: 99.9%. Isotypic Control (shaded): FITC Mouse IgG2b
References for Anti-MRP8 antibody [CF-145] (FITC) (ab112213)
This product has been referenced in:
Thacher SM et al. Cell type and gene-specific activity of the retinoid inverse agonist AGN 193109: divergent effects from agonist at retinoic acid receptor gamma in human keratinocytes. Cell Growth Differ10:255-62 (1999).
Read more (PubMed: 10319995) »
Klein ES et al. Identification and functional separation of retinoic acid receptor neutral antagonists and inverse agonists. J Biol Chem271:22692-6 (1996).
Read more (PubMed: 8798442) »