Anti-MRP8 + MRP14 antibody [27E10] (ab17050)

Overview

  • Product nameAnti-MRP8 + MRP14 antibody [27E10]
    See all MRP8 + MRP14 primary antibodies
  • Description
    Mouse monoclonal [27E10] to MRP8 + MRP14
  • SpecificityDoes not react with lymphocytes or platelets.
  • Tested applicationsSuitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Fr, IHC-Pmore details
    Unsuitable for: FuncS
  • Species reactivity
    Reacts with: Human, Rhesus monkey
    Does not react with: Mouse
  • Immunogen

    Human blood monocytes.

  • Epitopeab17050 recognises an epitope on the MRP8 + MRP14 complex which is not exposed on the individual subunits.
  • Positive control
    • Human granulocytes
  • General notes

    ab17050 can be used for early detection of inflammatory macrophages, characterisation of tumorous tissues and monitoring of peripheral blood cell cultures.

Properties

Applications

Our Abpromise guarantee covers the use of ab17050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/10. Perform under non-denaturing conditions only.
IP Use at an assay dependent concentration.
Flow Cyt 1/10. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/10.
IHC-P Use at an assay dependent concentration.
  • Application notesIs unsuitable for FuncS.
  • Target

    • RelevanceMRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • Alternative names
      • CAGA antibody
      • CAGB antibody
      • Calgranulin A antibody
      • Calgranulin B antibody
      • Calprotectin L1L subunit antibody
      • CFAG antibody
      • CP10 antibody
      • Cystic fibrosis antigen antibody
      • Leukocyte L1 complex light chain antibody
      • Macrophage L1 protein Calprotectin antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor related protein 8 antibody
      • MRP-14 antibody
      • MRP-8 antibody
      • P14 antibody
      • P8 antibody
      • S100 calcium binding protein A8 antibody
      • S100 calcium binding protein A9 antibody
      • S100A8 antibody
      • S100A9 antibody
      • Urinary stone protein band A antibody
      see all

    References for Anti-MRP8 + MRP14 antibody [27E10] (ab17050)

    This product has been referenced in:
    • Carrión M  et al. IL-22/IL-22R1 axis and S100A8/A9 alarmins in human osteoarthritic and rheumatoid arthritis synovial fibroblasts. Rheumatology (Oxford) 52:2177-86 (2013). Human . Read more (PubMed: 24056519) »
    • Iqbal SA  et al. Identification of fibrocytes from mesenchymal stem cells in keloid tissue: a potential source of abnormal fibroblasts in keloid scarring. Arch Dermatol Res 304:665-71 (2012). Read more (PubMed: 22407077) »

    See all 7 Publications for this product

    Product Wall

    Thank you for your enquiry.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any MRP8 and MRP14 antibody tested ...

    Read More

    Thank you for contacting us.

    The supplying lab send me the following information:

    Unfortunately we do not have a detailled protocol for use on paraffin sections. The application has been added in the past upon personal communicati...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Oral Dysplastic Epithelium cells)
    Specification Oral Dysplastic Epithelium cells
    Fixative Paraformaldehyde
    Antigen retrieval step Enzymatic
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2%
    Username

    Mr. Cancer center

    Verified customer

    Submitted Jul 25 2006

    For the generation of the antibody human blood monocytes are used. We regret to inform you that we have no more information about the immunogen. Furthermore we have no information about the reactivity with rat Calprotectin. Should you decide to go ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"