Overview

  • Product name
    Anti-MRP8 + MRP14 antibody [5.5]
    See all MRP8 + MRP14 primary antibodies
  • Description
    Mouse monoclonal [5.5] to MRP8 + MRP14
  • Tested applications
    Suitable for: IP, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation, acute monocytic leukaemia cells (Human).

  • Positive control
    • Neutrophil lysate (particularly neutrophils from spleen)

Properties

Applications

Our Abpromise guarantee covers the use of ab20222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight MRP8 10.8 kDa, MRP14 13 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    MRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
  • Cellular localization
    Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • CAGA antibody
    • CAGB antibody
    • Calgranulin A antibody
    • Calgranulin B antibody
    • Calprotectin L1L subunit antibody
    • CFAG antibody
    • CP10 antibody
    • Cystic fibrosis antigen antibody
    • Leukocyte L1 complex light chain antibody
    • Macrophage L1 protein Calprotectin antibody
    • Migration inhibitory factor related protein 14 antibody
    • Migration inhibitory factor related protein 8 antibody
    • MRP-14 antibody
    • MRP-8 antibody
    • P14 antibody
    • P8 antibody
    • S100 calcium binding protein A8 antibody
    • S100 calcium binding protein A9 antibody
    • S100A8 antibody
    • S100A9 antibody
    • Urinary stone protein band A antibody
    see all

References

This product has been referenced in:
  • Wang Y  et al. Platelet-derived S100 family member myeloid-related protein-14 regulates thrombosis. J Clin Invest 124:2160-71 (2014). WB, IHC ; Mouse . Read more (PubMed: 24691441) »
  • Hessian PA & Fisher L The heterodimeric complex of MRP-8 (S100A8) and MRP-14 (S100A9). Antibody recognition, epitope definition and the implications for structure. Eur J Biochem 268:353-63 (2001). Read more (PubMed: 11168370) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Cell lysate - whole cell (Fibroblasts)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Fibroblasts
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Ms. Asmaa Alsousi

Verified customer

Submitted Dec 20 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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