Recombinant MRP8/14 heterocomplex (Human). The antigen is produced by the Ca2+ dependent heterocomplex formation of MRP8 (S100A8 or Calgranulin A) and MRP14 (S100A9 or Calgranulin B), two calcium binding proteins of the S 100 protein family.
General notesPeripheral blood monocytes carry the antigen extra and intracellularly, neutrophils only intracellularly. The antigen is present on macrophages in the red pulp of the spleen and in the liver. It is also strongly expressed on macrophages in acute inflamed tissues (peridontitis, contact excema, urticaria, erythrodermia).
In immune histochemistry, this antibody detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Purification notesPurified from egg yolk by repeated precipitation.
Primary antibody notesIn immune histochemistry, this antibody detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml.
Use a concentration of 4 µg/ml.
1/250. Proteinase K pretreatment for antigen retrieval is recommended.
RelevanceMRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
ab51863 at 4µg/ml staining human spleen; paraffin embedded.
References for Anti-MRP8 + MRP14 antibody (Biotin) (ab51863)
This product has been referenced in:
Lood C et al. Protein synthesis of the pro-inflammatory S100A8/A9 complex in plasmacytoid dendritic cells and cell surface S100A8/A9 on leukocyte subpopulations in systemic lupus erythematosus. Arthritis Res Ther13:R60 (2011).
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