The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Ready to use.
For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceMRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
MRP8 + MRP14 staining in human tonsil by Immunohistochemistry. Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of macrophages.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 + MRP14 antibody [MAC 387], prediluted (ab75636)This image is a courtesy of Anonymous Abreview
ab75636 staining MRP8 + MRP14 in human tonsil and colon tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissues underwent formaldehyde fixation before heat mediated antigen retrieval with citrate at 1000C for 20 minutes. The primary antibody was used diluted to 1 µg/ml with commercially available background reducing agents and incubated with sample for 20 minutes at 25°C. A HRP conjugated rabbit polyclonal to mouse IgG was used undiluted as secondary antibody.