Anti-MRP8 + MRP14 antibody [MAC 387], prediluted (ab75636)


  • Product nameAnti-MRP8 + MRP14 antibody [MAC 387], prediluted
    See all MRP8 + MRP14 primary antibodies
  • Description
    Mouse monoclonal [MAC 387] to MRP8 + MRP14, prediluted
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Cat, Dog, Human, Pig, Monkey, Baboon
  • Immunogen

    Affinity purified monocyte membrane preparation

  • Positive control
    • Tonsil, lymph node, or spleen tissues.



Our Abpromise guarantee covers the use of ab75636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-P: Ready to use.
    For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37C.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • Alternative names
      • CAGA antibody
      • CAGB antibody
      • Calgranulin A antibody
      • Calgranulin B antibody
      • Calprotectin L1L subunit antibody
      • CFAG antibody
      • CP10 antibody
      • Cystic fibrosis antigen antibody
      • Leukocyte L1 complex light chain antibody
      • Macrophage L1 protein Calprotectin antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor related protein 8 antibody
      • MRP-14 antibody
      • MRP-8 antibody
      • P14 antibody
      • P8 antibody
      • S100 calcium binding protein A8 antibody
      • S100 calcium binding protein A9 antibody
      • S100A8 antibody
      • S100A9 antibody
      • Urinary stone protein band A antibody
      see all

    Anti-MRP8 + MRP14 antibody [MAC 387], prediluted images

    • MRP8 + MRP14 staining in human tonsil by Immunohistochemistry. Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of macrophages.
    • ab75636 staining MRP8 + MRP14 in human tonsil and colon tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissues underwent formaldehyde fixation before heat mediated antigen retrieval with citrate at 1000C for 20 minutes. The primary antibody was used diluted to 1 µg/ml with commercially available background reducing agents and incubated with sample for 20 minutes at 25°C. A HRP conjugated rabbit polyclonal to mouse IgG was used undiluted as secondary antibody.

      See Abreview

    References for Anti-MRP8 + MRP14 antibody [MAC 387], prediluted (ab75636)

    ab75636 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Tonsil, colon)
    Specification Tonsil, colon
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate at 100C for 20 minutes
    Permeabilization No

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    Submitted Jun 02 2009