Overview

  • Product name
    Anti-MRPL12 antibody
  • Description
    Rabbit polyclonal to MRPL12
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-198 of MRPL12 (P52815).

  • Positive control
    • A431, H1299, HeLa, HepG2 and Mouse brain whole cell lysates; Human breast carcinoma tissue, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab154961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-MRPL12 antibody (ab154961) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 21 kDa
  • Anti-MRPL12 antibody (ab154961) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 21 kDa
  • Immunofluorescence analysis of methanol fixed HeLa cells labeling MRPL12 with ab154961 at 1/200. Lower panel shows costaining with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling MRPL12 with ab154961 at 1/250.

References

ab154961 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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