ICC/IF image of ab58334 stained CaCo2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58334 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC-P - MRPL12 antibody (ab58334)
MRPL12 antibody (ab58334) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human breast cancer tissue.
Western blot - MRPL12 antibody (ab58334)
Predicted band size : 21 kDa MRPL12 antibody (ab58334) at 1ug/lane + COLO cell lysate at 25ug/lane.
Flow Cytometry-Anti-MRPL12 antibody(ab58334)
Overlay histogram showing HepG2 cells stained with ab58334 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58334, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-MRPL12 antibody (ab58334)
This product has been referenced in:
Rorbach J et al. MRM2 and MRM3 are involved in biogenesis of the large subunit of the mitochondrial ribosome. Mol Biol Cell25:2542-55 (2014).
Read more (PubMed: 25009282) »
Rorbach J et al. C7orf30 is necessary for biogenesis of the large subunit of the mitochondrial ribosome. Nucleic Acids Res : (2012).
Read more (PubMed: 22238376) »