Overview

  • Product name
  • Description
    Mouse polyclonal to MRPL13
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length MRPL13 protein (Human)

  • Positive control
    • MRPL13 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).

Target

  • Relevance
    Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [Entrez Gene]
  • Cellular localization
    Mitochondrion
  • Database links
  • Alternative names
    • 39S ribosomal protein L13, mitochondrial antibody
    • L13 antibody
    • L13A antibody
    • L13mt antibody
    • Mitochondrial ribosomal protein L13 antibody
    • MRP L13 antibody
    • OTTHUMP00000228247 antibody
    • OTTHUMP00000228248 antibody
    • RPL13 antibody
    • RPML13 antibody
    see all

Images

  • All lanes : Anti-MRPL13 antibody (ab68239) at 1/500 dilution

    Lane 1 : MRPL13 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 21 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

References

ab68239 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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