Overview

  • Product name
  • Description
    Mouse polyclonal to MRPL18
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    MRPL18 (NP_054880.2, 1 a.a. ~ 180 a.a) full-length human protein.

  • Positive control
    • Human liver tissue lysate, transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa).

Target

  • Sequence similarities
    Belongs to the ribosomal protein L18P family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 39S ribosomal protein L18 antibody
    • 39S ribosomal protein L18 mitochondrial antibody
    • 39S ribosomal protein L18, mitochondrial precursor antibody
    • HSPC071 antibody
    • L18mt antibody
    • mitochondrial antibody
    • Mitochondrial ribosomal protein L18 antibody
    • Mitochondrial ribosomal protein L18 (MRPL18 protein) antibody
    • MRP-L18 antibody
    • Mrpl18 antibody
    • RM18_HUMAN antibody
    • RP1 195P10.3 001 antibody
    see all

Images

  • Anti-MRPL18 antibody (ab67844) at 1/500 dilution + Human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 21 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-MRPL18 antibody (ab67844) at 1/500 dilution

    Lane 1 : MRPL18 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 21 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 21 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Smirnov A  et al. Biological significance of 5S rRNA import into human mitochondria: role of ribosomal protein MRP-L18. Genes Dev 25:1289-305 (2011). WB ; Human . Read more (PubMed: 21685364) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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