Recombinant
RabMAb

Anti-MRPL28 antibody [EPR7578(B)] (ab126719)

Overview

  • Product name
    Anti-MRPL28 antibody [EPR7578(B)]
    See all MRPL28 primary antibodies
  • Description
    Rabbit monoclonal [EPR7578(B)] to MRPL28
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human MRPL28 (Q13084).

  • Positive control
    • Fetal kidney cell lysates JAR cell lysates HepG2 cell lysates A549 cell lysates 293T cell lysates A549 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IP 1/100 - 1/500.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Tissue specificity
      Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue.
    • Sequence similarities
      Belongs to the ribosomal protein L28P family.
    • Cellular localization
      Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • 39S ribosomal protein L28 antibody
      • 39S ribosomal protein L28 mitochondrial antibody
      • 39S ribosomal protein L28 mitochondrial precursor antibody
      • HGNC6756 antibody
      • L28mt antibody
      • MAAT 1 antibody
      • MAAT1 antibody
      • Melanoma antigen p15 antibody
      • Melanoma associated antigen recognised by cytotoxic T lymphocytes antibody
      • Melanoma associated antigen recognized by T lymphocytes antibody
      • Melanoma-associated antigen recognized by T-lymphocytes antibody
      • MGC8499 antibody
      • mitochondrial antibody
      • Mitochondrial ribosomal protein L28 antibody
      • MRP L28 antibody
      • MRP-L28 antibody
      • MRPL 28 antibody
      • mrpl28 antibody
      • P15 antibody
      • RM28_HUMAN antibody
      see all

    Images

    • All lanes : Anti-MRPL28 antibody [EPR7578(B)] (ab126719) at 1/1000 dilution

      Lane 1 : Fetal kidney cell lysates
      Lane 2 : JAR cell lysates
      Lane 3 : HepG2 cell lysates
      Lane 4 : A549 cell lysates
      Lane 5 : 293T cell lysates


      Predicted band size : 30 kDa
    • ab126719, at 1/250 dilution, stains MRPL28 in A549 cells by immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126719 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    6.5 µg
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Sample
    Human Cell lysate - whole cell (HepG2)
    Specification
    HepG2
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 26 2015

    Thank you for your reply. I am glad I could help you choose the best antibody for your experiment.

    We do not have any distributor in Norway, you can order directly from us. Please let me know if you need a proforma and I will be happy to send...

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    Thank you for contacting us.

    There are four antibodies in our catalogue suitable for WB in either one of the mentioned species.

    Product ab126719 is a rabbit monoclonal that has been validated by WB in Human, Mouse and Rat, you can h...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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