Overview

  • Product name
  • Description
    Goat polyclonal to MRPL3
  • Tested applications
    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    NVCQPGAPSITFA

    , corresponding to C terminal amino acids 336-348 of Human MRPL3

  • Positive control
    • This antibody gave a positive result when used in the following methanol fixed cell lines: Caco-2

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration. PubMed: 20144953

This antibody gave a positive result in ELISA against the immunizing peptide. Antibody detection limit dilution >1:32,000.

WB Use at an assay dependent concentration.

Target

  • Sequence similarities
    Belongs to the ribosomal protein L3P family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 39S ribosomal protein L3 antibody
    • 39S ribosomal protein L3 mitochondrial antibody
    • COXPD9 antibody
    • L3mt antibody
    • Mitochondrial 39S ribosomal protein L3 antibody
    • Mitochondrial 60S ribosomal protein L3 antibody
    • mitochondrial antibody
    • mitochondrial ribosomal protein antibody
    • Mitochondrial ribosomal protein L3 antibody
    • MRL3 antibody
    • MRP-L3 antibody
    • MRPL3 antibody
    • RM03_HUMAN antibody
    • RPML3 antibody
    see all

Anti-MRPL3 antibody images

  • ab39268 stained Caco-2 cells. The cells were 100% methamol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39268 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was 150133 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

References for Anti-MRPL3 antibody (ab39268)

This product has been referenced in:
  • Zaganelli S  et al. The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. J Biol Chem 292:4519-4532 (2017). Read more (PubMed: 28082677) »
  • Rorbach J  et al. Human mitochondrial ribosomes can switch their structural RNA composition. Proc Natl Acad Sci U S A 113:12198-12201 (2016). WB . Read more (PubMed: 27729525) »

See all 9 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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