ab39268 stained Caco-2 cells. The cells were 100% methamol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39268 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was 150133 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
References for Anti-MRPL3 antibody (ab39268)
This product has been referenced in:
Rorbach J et al. MRM2 and MRM3 are involved in biogenesis of the large subunit of the mitochondrial ribosome. Mol Biol Cell25:2542-55 (2014).
Read more (PubMed: 25009282) »
Rorbach J et al. C7orf30 is necessary for biogenesis of the large subunit of the mitochondrial ribosome. Nucleic Acids Res : (2012).
Read more (PubMed: 22238376) »