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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MRPL48 aa 1-100. The exact sequence is proprietary.
Database link: Q96GC5
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab194826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 24 kDa).|
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling MRPL48 with ab194826 at 1/150 dilution. A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab194826. Cytoplasm staining on human cervix carcinoma tissue was observed.
Immunofluorescent analysis of A431 cells labeling MRPL48 with ab194826 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on HepG2 cell line was observed.
Flow cytometry analysis of HeLa cells labelling MRPL48 (red) with purified ab194826 at dilution of 1/60. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab194826 at 1/30 dilution (lane 1) or PBS control (lane 2)
For Western blot: ab194826 was used at 1/1000 dilution (0.6µg/ml). Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
ab194826 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"