Overview

  • Product nameAnti-MRPL52 antibody
  • Description
    Rabbit polyclonal to MRPL52
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 26-123 of Human MRPL52 (Q86TS9).

  • Positive control
    • RT-4 and U-251 MG, NIH-3T3 and NBT-II cell lysates, Human pancreas tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG

Applications

Our Abpromise guarantee covers the use of ab121366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 14 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-MRPL52 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab121366 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-MRPL52 antibody (ab121366) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma cell lysate
    Lane 4 : Human Liver cell lysate
    Lane 5 : Human Tonsil cell lysate


    Predicted band size : 14 kDa
  • All lanes : Anti-MRPL52 antibody (ab121366) at 1/250 dilution

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : NBT-II cell lysate


    Predicted band size : 14 kDa
  • Immunohistochemical analysis of paraffin embedded Human pancreas tissue labelling MRPL52 with ab121366 antibody at a dilution of 1/50.

References for Anti-MRPL52 antibody (ab121366)

ab121366 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"