Overview

  • Product name
  • Description
    Rabbit polyclonal to MRPS10
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-178 of Human MRPS10 (AAH12560).

  • Positive control
    • NT2D1, IMR32, U-87 MG and MCF7 cells and whole cell lysates; U373 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab111715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0)
ICC/IF 1/100 - 1/500.

Target

  • Relevance
    Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S10P family. Pseudogenes corresponding to this gene are found on chromosomes 1q, 3p, and 9p. [Entrez Gene]
  • Cellular localization
    Mitochondrion
  • Database links
  • Alternative names
    • Mitochondrial 28S ribosomal protein S10 antibody
    • MRP S10 antibody
    • PNAS 122 antibody
    • S10mt antibody

Images

  • Anti-MRPS10 antibody (ab111715) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size : 23 kDa
  • ab111715 at 1/200 dilution staining MRPS10 in methanol-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
  • ab111715 at 1/500 dilution staining MRPS10 in Formalin-fixed, paraffin-embedded U373 xenograft tissue by Immunohistochemistry.

References

ab111715 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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