Recombinant Anti-MRPS18B antibody [EPR19298] (ab191891)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19298] to MRPS18B
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MRPS18B antibody [EPR19298]
See all MRPS18B primary antibodies -
Description
Rabbit monoclonal [EPR19298] to MRPS18B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver, fetal heart and fetal kidney lysates; Mouse heart, kidney and spleen lysates; and Rat kidney and liver lysates. MCF7, HeLa, Jurkat, HepG2, C6, RAW 264.7, NIH/3T3 whole cell lysates. ICC/IF: HeLa and MCF7 cell lines. IP: MCF7 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19298 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000.
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IP |
1/40.
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WB |
1/2000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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Notes |
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ICC/IF
1/1000. |
IP
1/40. |
WB
1/2000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
Target
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Sequence similarities
Belongs to the ribosomal protein S18P family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 28973 Human
- Entrez Gene: 66973 Mouse
- Omim: 611982 Human
- SwissProt: Q9Y676 Human
- SwissProt: Q99N84 Mouse
- Unigene: 655329 Human
- Unigene: 29484 Mouse
- Unigene: 440735 Mouse
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Alternative names
- 28S ribosomal protein S18-2 antibody
- 28S ribosomal protein S18b antibody
- 28S ribosomal protein S18b, mitochondrial antibody
see all
Images
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All lanes : Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 3 minutes5% NFDM/TBST: Blocking and dilution buffer.
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All lanes : Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 3 minutes5% NFDM/TBST: Blocking and dilution buffer.
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All lanes : Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 3 minutes5% NFDM/TBST: Blocking and dilution buffer.
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All lanes : Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 15 seconds5% NFDM/TBST: Blocking and dilution buffer.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling MRPS18B with ab191891 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HeLa cell line.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
1. ab191891 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
2. ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution. -
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cell line labeling MRPS18B with ab191891 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on MCF7 cell line.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
1. ab191891 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
2. ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution. -
MRPS18B was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab191891 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab191891 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: MCF7 whole cell lysate, 10ug (Input).
Lane 2: ab191891 IP in MCF7 whole cell lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab191891 in MCF7 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab191891 has not yet been referenced specifically in any publications.