Recombinant
RabMAb

Anti-MRPS18B antibody [EPR19298] (ab191891)

Overview

  • Product name
    Anti-MRPS18B antibody [EPR19298]
    See all MRPS18B primary antibodies
  • Description
    Rabbit monoclonal [EPR19298] to MRPS18B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Human MRPS18B aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y676

  • Positive control
    • WB: Human fetal liver, fetal heart and fetal kidney lysates; Mouse heart, kidney and spleen lysates; and Rat kidney and liver lysates. MCF7, HeLa, Jurkat, HepG2, C6, RAW 264.7, NIH/3T3 whole cell lysates. ICC/IF: HeLa and MCF7 cell lines. IP: MCF7 whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
IP 1/40.
WB 1/2000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

Target

  • Sequence similarities
    Belongs to the ribosomal protein S18P family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 28S ribosomal protein S18-2 antibody
    • 28S ribosomal protein S18b antibody
    • 28S ribosomal protein S18b, mitochondrial antibody
    • C6orf14 antibody
    • DKFZp564H0223 antibody
    • HSPC 183 antibody
    • HSPC183 antibody
    • mitochondrial antibody
    • Mitochondrial ribosomal protein S18 2 antibody
    • Mitochondrial ribosomal protein S18B antibody
    • MRP S18 2 antibody
    • MRP S18 b antibody
    • MRP S18b antibody
    • MRP-S18-2 antibody
    • MRP-S18-b antibody
    • MRPS 18B antibody
    • MRPS18 2 antibody
    • Mrps18-b antibody
    • MRPS18B antibody
    • PTD017 antibody
    • RT18B_HUMAN antibody
    • S18amt antibody
    • S18mt-b antibody
    see all

Images

  • All lanes : Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and dilution buffer.

  • All lanes : Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and dilution buffer.

  • All lanes : Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat kidney lysate
    Lane 5 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and dilution buffer.

  • All lanes : Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 15 seconds


    5% NFDM/TBST: Blocking and dilution buffer.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling MRPS18B with ab191891 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    1. ab191891 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
    2. ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cell line labeling MRPS18B with ab191891 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on MCF7 cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    1. ab191891 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
    2. ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • MRPS18B was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab191891 at 1/40 dilution.
    Western blot was performed from the immunoprecipitate using ab191891 at 1/1000 dilution.
    VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
    Lane 1: MCF7 whole cell lysate, 10ug (Input).
    Lane 2: ab191891 IP in MCF7 whole cell lysate.
    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab191891 in MCF7 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

References

ab191891 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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