Overview

  • Product nameAnti-MRPS21 antibody
  • Description
    Rabbit polyclonal to MRPS21
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    synthetic peptide derived from internal of human MRPS21.

  • Positive control
    • human pancreas tissue, extracts from COS cells, MCF-7 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab74101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 11 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein. [provided by RefSeq]
  • Cellular localizationMitochondrial small ribosomal subunit
  • Database links
  • Alternative names
    • MDS016 antibody
    • mitochondrial 28S ribosomal protein S21 antibody
    • Mitochondrial ribosomal protein S21 antibody
    • MRP S21 antibody
    • S21mt antibody
    see all

Anti-MRPS21 antibody images

  • All lanes : Anti-MRPS21 antibody (ab74101) at 1/500 dilution

    Lane 1 : extracts from COS cells
    Lane 2 : extracts from COS cells with immunizing peptide at 5 µg

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 11 kDa
    Observed band size : 11 kDa
  • Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using 1/50 ab74101 with (right panel) or without (left panel) preincubation with immunizing peptide.
  • Immunofluorescence analysis of MCF-7 cells, using 1/500 ab74101 with (right panel) or without (left panel) preincubation with immunizing peptide.

References for Anti-MRPS21 antibody (ab74101)

ab74101 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"