The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. This gene lies adjacent to and downstream of the gonadotropin-releasing hormone precursor gene.
Mitochondrion: mitochondrial small ribosomal subunit
Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling MRPS26 with ab181863 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Western blot - Anti-MRPS26 [EPR14396] antibody (ab181863)
All lanes : Anti-MRPS26 antibody [EPR14396] (ab181863) at 1/2000 dilution
Lane 1 : HepG2 cell lysate Lane 2 : HeLa cell lysate Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling MRPS26 with ab181863 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary at 1/200 dilution and counter-stained with DAPI (blue).