Recombinant
RabMAb

Anti-MRPS26 antibody [EPR14396] (ab181863)

Overview

  • Product name
    Anti-MRPS26 antibody [EPR14396]
    See all MRPS26 primary antibodies
  • Description
    Rabbit monoclonal [EPR14396] to MRPS26
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human MRPS26 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BYN8

  • Positive control
    • HeLa, HepG2 and Raji cell lysates; HeLa and HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab181863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ICC/IF 1/250.

Target

  • Relevance
    Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. This gene lies adjacent to and downstream of the gonadotropin-releasing hormone precursor gene.
  • Cellular localization
    Mitochondrion: mitochondrial small ribosomal subunit
  • Database links
  • Alternative names
    • 28S ribosomal protein S13 mitochondrial antibody
    • 28S ribosomal protein S26 mitochondrial antibody
    • C20orf193 antibody
    • dJ534B8.3 antibody
    • GI008 antibody
    • Mitochondrial ribosomal protein S26 antibody
    • MRP S13 antibody
    • MRP S26 antibody
    • MRPS13 antibody
    • NY BR 87 antibody
    • RPMS13 antibody
    • S13mt antibody
    • S26mt antibody
    • Serologically defined breast cancer antigen NY BR 87 antibody
    see all

Images

  • Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling MRPS26 with ab181863 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • All lanes : Anti-MRPS26 antibody [EPR14396] (ab181863) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 24 kDa
  • Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling MRPS26 with ab181863 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

References

ab181863 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181863.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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