Overview

  • Product name
    Anti-MRPS27 antibody
  • Description
    Rabbit polyclonal to MRPS27
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-153 of Human MRPS27 (UniProt: Q92552).

  • Positive control
    • A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells; Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Sequence similarities
    Contains 2 PPR (pentatricopeptide) repeats.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 28S ribosomal protein S27 antibody
    • 28S ribosomal protein S27, mitochondrial antibody
    • KIAA0264 antibody
    • Mitochondrial 28S ribosomal protein S27 antibody
    • mitochondrial antibody
    • mitochondrial ribosomal protein S27 antibody
    • MRP-S27 antibody
    • MRPS27 antibody
    • RT27_HUMAN antibody
    • S27mt antibody
    see all

Images

  • Anti-MRPS27 antibody (ab153940) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 48 kDa



    12% SDS PAGE
  • Immunohistochemistry analysis of paraffin-embedded Human Colon carcinoma tissue labeling MRPS27 with ab153940 at 1/250 dilution.
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling MRPS27 with ab153940 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

ab153940 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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