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Rabbit polyclonal to MRPS27
Recombinant fragment, corresponding to a region within amino acids 1-153 of Human MRPS27 (UniProt: Q92552).
- A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells; Human colon carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 48 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/100 - 1/1000.
Contains 2 PPR (pentatricopeptide) repeats.
Information by UniProt
- 28S ribosomal protein S27 antibody
- 28S ribosomal protein S27, mitochondrial antibody
- KIAA0264 antibody
Western blot - Anti-MRPS27 antibody (ab153940)
Anti-MRPS27 antibody (ab153940) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 48 kDa
12% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS27 antibody (ab153940)
Immunohistochemistry analysis of paraffin-embedded Human Colon carcinoma tissue labeling MRPS27 with ab153940 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MRPS27 antibody (ab153940)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling MRPS27 with ab153940 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"