Recombinant
RabMAb

Anti-MRRF antibody [EPR14140(B)] (ab181223)

Overview

  • Product name
    Anti-MRRF antibody [EPR14140(B)]
    See all MRRF primary antibodies
  • Description
    Rabbit monoclonal [EPR14140(B)] to MRRF
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MRRF aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96E11

  • Positive control
    • Human cardiac muscle tissue. HeLa, 293, A431 and HepG2 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR14140(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.

Target

  • Function
    Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.
  • Sequence similarities
    Belongs to the RRF family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2400002D02Rik antibody
    • MGC93722 antibody
    • mitochondrial antibody
    • mitochondrial ribosome recycling factor antibody
    • MRFF antibody
    • Mrrf antibody
    • MTRRF antibody
    • OTTHUMP00000022037 antibody
    • OTTMUSP00000013027 antibody
    • Ribosome recycling factor, mitochondrial antibody
    • Ribosome recycling factor, mitochondrial precursor antibody
    • Ribosome-recycling factor antibody
    • Ribosome-releasing factor antibody
    • Ribosome-releasing factor, mitochondrial antibody
    • RP23-324A16.1 antibody
    • RRF antibody
    • RRFM_HUMAN antibody
    see all

Images

  • All lanes : Anti-MRRF antibody [EPR14140(B)] (ab181223) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 29 kDa

    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemical analysis of paraffin embedded Human cardiac muscle tissue sections labeling MRRF using ab181223 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Immunohistochemical analysis of paraffin embedded Human kidney tissue sections labeling MRRF using ab181223 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

References

ab181223 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rat Cell lysate - whole cell (skeletal muscle)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Jan 15 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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