Overview

  • Product name
  • Description
    Rabbit polyclonal to MS4A4A
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide between amino acids 40-68 from the N terminal region of Human MS4A4A (NP_076926.2).

  • Positive control
    • MDA-MB435 cell line lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: U2OS.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab107543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 25 kDa.

Target

  • Relevance
    MS4A4A is a member of the membrane spanning 4A gene family. This nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and non lymphoid tissues. MS4A4A may be involved in signal transduction as a component of a multimeric receptor complex. There are two named isoforms.
  • Cellular localization
    Cell Membrane; Multi pass membrane protein.
  • Database links
  • Alternative names
    • 4SPAN1 antibody
    • CD20 antigen like 1 antibody
    • CD20L1 antibody
    • MS4A4 antibody

Images

  • ICC/IF image of ab107543 stained U2OS cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107543 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-MS4A4A antibody (ab107543) at 1/100 dilution + MDA-MB435 cell line lysate at 35 µg

    Predicted band size : 25 kDa

References

ab107543 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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