Overview

  • Product nameAnti-MSF antibody
    See all MSF primary antibodies
  • Description
    Rabbit polyclonal to MSF
  • SpecificityThis antibody is specific for MSF (C term).
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the C-terminal region of human MSF.

  • Positive control
    • Hepatocarcinoma tissue and Jurkat cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab38314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.
IHC-P 1/25 - 1/100.
WB 1/100 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 64 kDa).

Target

  • FunctionFilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
  • Tissue specificityWidely expressed. Isoforms are differentially expressed in testes, kidney, liver heart, spleen, brain, peripheral blood leukocytes, skeletal muscle and kidney. Specific isoforms appear to demonstrate tissue specificity. Isoform 5 is the most highly expressed in fetal tissue. Isoform 1 is detected in all tissues except the brain and thymus, while isoform 2, isoform 3, and isoform 4 are detected at low levels in approximately half of the fetal tissues.
  • Involvement in diseaseNote=A chromosomal aberration involving SEPT9/MSF is found in therapy-related acute myeloid leukemia (t-AML). Translocation t(11;17)(q23;q25) with MLL.
    Defects in SEPT9 are a cause of hereditary neuralgic amyotrophy (HNA) [MIM:162100]; also known as neuritis with brachial predilection (NAPB) or hereditary brachial plexus neuropathy or hereditary neuralgic amyotrophy with predilection for brachial plexus. HNA is an autosomal dominant form of recurrent focal neuropathy characterized clinically by acute, recurrent episodes of brachial plexus neuropathy with muscle weakness and atrophy preceded by severe pain in the affected arm. HNA is triggered by environmental factors such as infection or parturition.
  • Sequence similaritiesBelongs to the septin family.
  • Cellular localizationCytoplasm > cytoskeleton. In an epithelial cell line, concentrates at cell-cell contact areas. After TGF-beta1 treatment and induction of epithelial to mesenchymal transition, colocalizes partly with actin stress fibers. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF17q25 antibody
    • Cell division control protein septin D1 antibody
    • KIAA0991 antibody
    • MLL septin like fusion protein antibody
    • MLL septin-like fusion protein antibody
    • MLL septin-like fusion protein MSF-A antibody
    • MSF antibody
    • MSF1 antibody
    • NAPB antibody
    • Ov/Br septin antibody
    • Ovarian/breast septin alpha antibody
    • Ovarian/Breast septin antibody
    • PNUTL4 antibody
    • SEPT9 antibody
    • SEPT9_HUMAN antibody
    • SeptD1 antibody
    • Septin 9 antibody
    • Septin D1 antibody
    • Septin-9 antibody
    • SINT1 antibody
    see all

Anti-MSF antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue sections labeling MSF with ab38314 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • ICC/IF image of ab38314 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38314, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-MSF antibody (ab38314) at 1/1000 dilution + Jurkat whole cell lysate at 35 µg/ml

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 64 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 and 40 kDa (possible degradation product).

    Blocking/Dilution buffer: 5% NFDM/TBST.

References for Anti-MSF antibody (ab38314)

This product has been referenced in:
  • Akil A  et al. Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV. Nat Commun 7:12203 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27417143) »

See 1 Publication for this product

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