Overview

  • Product nameAnti-MSF antibody
    See all MSF primary antibodies
  • Description
    Mouse polyclonal to MSF
  • SpecificityReactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human MSF protein (NP_006631).

  • Positive control
    • HepG2 cell lysate; MSF transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab89160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 65 kDa.

Target

  • FunctionFilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
  • Tissue specificityWidely expressed. Isoforms are differentially expressed in testes, kidney, liver heart, spleen, brain, peripheral blood leukocytes, skeletal muscle and kidney. Specific isoforms appear to demonstrate tissue specificity. Isoform 5 is the most highly expressed in fetal tissue. Isoform 1 is detected in all tissues except the brain and thymus, while isoform 2, isoform 3, and isoform 4 are detected at low levels in approximately half of the fetal tissues.
  • Involvement in diseaseNote=A chromosomal aberration involving SEPT9/MSF is found in therapy-related acute myeloid leukemia (t-AML). Translocation t(11;17)(q23;q25) with MLL.
    Defects in SEPT9 are a cause of hereditary neuralgic amyotrophy (HNA) [MIM:162100]; also known as neuritis with brachial predilection (NAPB) or hereditary brachial plexus neuropathy or hereditary neuralgic amyotrophy with predilection for brachial plexus. HNA is an autosomal dominant form of recurrent focal neuropathy characterized clinically by acute, recurrent episodes of brachial plexus neuropathy with muscle weakness and atrophy preceded by severe pain in the affected arm. HNA is triggered by environmental factors such as infection or parturition.
  • Sequence similaritiesBelongs to the septin family.
  • Cellular localizationCytoplasm > cytoskeleton. In an epithelial cell line, concentrates at cell-cell contact areas. After TGF-beta1 treatment and induction of epithelial to mesenchymal transition, colocalizes partly with actin stress fibers. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF17q25 antibody
    • Cell division control protein septin D1 antibody
    • KIAA0991 antibody
    • MLL septin like fusion protein antibody
    • MLL septin-like fusion protein antibody
    • MLL septin-like fusion protein MSF-A antibody
    • MSF antibody
    • MSF1 antibody
    • NAPB antibody
    • Ov/Br septin antibody
    • Ovarian/breast septin alpha antibody
    • Ovarian/Breast septin antibody
    • PNUTL4 antibody
    • SEPT9 antibody
    • SEPT9_HUMAN antibody
    • SeptD1 antibody
    • Septin 9 antibody
    • Septin D1 antibody
    • Septin-9 antibody
    • SINT1 antibody
    see all

Anti-MSF antibody images

  • Anti-MSF antibody (ab89160) at 1/500 dilution + HepG2 cell lysate at 50 µg

    Predicted band size : 65 kDa
    Observed band size : ~65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 73 kDa,76 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-MSF antibody (ab89160) at 1/500 dilution

    Lane 1 : MSF transfected 293T lysate
    Lane 2 : Non transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 65 kDa

References for Anti-MSF antibody (ab89160)

ab89160 has not yet been referenced specifically in any publications.

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