The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
FunctionComponent of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
Involvement in diseaseDefects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
Sequence similaritiesBelongs to the DNA mismatch repair mutS family. MSH3 subfamily.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
All lanes : Anti-MSH3 antibody (ab69619) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells Lane 2 : Extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 127 kDa Observed band size : 127 kDa Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
References for Anti-MSH3 antibody (ab69619)
This product has been referenced in:
Zhao J et al. Mismatch repair and nucleotide excision repair proteins cooperate in the recognition of DNA interstrand crosslinks. Nucleic Acids Res37:4420-9 (2009).
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