Overview

  • Product name
    Anti-MSH3 antibody - C-terminal
    See all MSH3 primary antibodies
  • Description
    Rabbit polyclonal to MSH3 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant protein fragment, corresponding to a region within C terminal amino acids 916-1137 of Human MSH3 (UniProt ID: P20585).

  • Positive control
    • A549 cell lysate; HeLa cells; Human colon carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 127 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
  • Involvement in disease
    Defects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
  • Sequence similarities
    Belongs to the DNA mismatch repair mutS family. MSH3 subfamily.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • Divergent upstream protein antibody
    • DNA mismatch repair protein antibody
    • DNA mismatch repair protein Msh 3 antibody
    • DNA mismatch repair protein MSH3 antibody
    • DUC 1 antibody
    • DUC1 antibody
    • DUG antibody
    • DUP antibody
    • hMSH3 antibody
    • MGC163306 antibody
    • MGC163308 antibody
    • Mismatch repair protein 1 antibody
    • MRP 1 antibody
    • MRP1 antibody
    • MSH 3 antibody
    • MSH3 antibody
    • MSH3_HUMAN antibody
    • MutS homolog 3 (E. coli) antibody
    • MutS homolog 3 antibody
    see all

Images

  • Anti-MSH3 antibody - C-terminal (ab154521) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 127 kDa



    5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling MSH3 using ab154521 at a 1/500 dilution. image in the lower panel was costained with Hoechst 33342.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue, labeling MSH3 using ab154521 at a 1/500 dilution.

References

ab154521 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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